Özet:

Aim: Research has shown that the increased use of chemicals which are offered to consumers, sometimes without sufficient testing, has impacted human health and natural resources negatively. For this reason, many researchers have developed cost-effective test systems which can examine the carcinogenic and mutagenic activity of chemicals in a short time. Ames is an example of such a test system which can detect mutations at cellular level. Also the Ames test is accepted as a reliable method for specifically testing the toxic and mutagenic-carcinogenic effects of raw materials to be used in pharmaceuticals. Material and Methods: In this study, the mutagenic and carcinogenic effect of methylphenidate, that is used for the treatment of Attention-Deficit Hyperactivity Disorder, was investigated by the Ames Salmonella/microsome test in the absence and presence of metabolic activation. The Ames Salmonella/microsome test can play an important role to identify the mutagenic effects of carcinogenic compounds and is used for the research and development of drugs. TA 98 and TA 100 strains were used in experiments. TA 98 is designed for frame-shift mutagens and TA 100 is designed for base-pair mutagens. The mutagenic activity was screened in two groups with or without S9 metabolic activation. The results were evaluated and the mean values were compared with positive and negative controls. Results: According to the results of experiments conducted with TA98 and TA100, revertant colony count was not found higher than those spontaneous revertant colony count among all of the concentrations and as well as under the conditions of with and without S9 (p>0.05). Conclusion: In conclusion, it was shown that methylphenidate was non-mutagenic for TA 98 and TA 100 with or without S9 metabolic activation.

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