Özet:

The current work highlights the use of a molecular technique to identify the etiological agent of localized cutaneous Leishmaniasis (Leishmania spp. parasites) in Iraqi patients with clinical manifestations of suspected Leishmaniasis. Twelve participants were recruited for this study between January 2021 and June 2021 at Baghdad hospital's external clinics. According to the manufacturer's instructions, the acquired samples in the form of skin biopsies were directly treated to a DNA isolation technique employing total DNA using the GeneJET Genomic DNA purification kit. Real-time polymerase chain reaction (real-time –PCR) was used to confirm the presence of Leishmania spp. in the twelve samples. Eight of twelve DNA samples (8/12= 66.66%)  were positive for the Leishmania suspect pathogen, as determined by positive real-time PCR results. The current findings support the ability of the molecular detection approach used in this work to detect and confirm the presence of Leishmania spp. pathogen in cutaneous Leishmaniasis in a quick, accurate, specific, and sensitive manner.

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