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Amaç: Genişlemiş spektrumlu beta-laktamaz (GSBL) enzimi üretimi ile beta-laktam antibiyotiklere direnç gelişmesi E.coli ve Klebsiella species( Klebsiella spp.) suşlarında en sık görülen direnç mekanizmasıdır. GBSL üreten E. coli ve Klebsiella spp. neden olduğu enfeksiyonların tedavisinde son yıllarda temosilin ve fosfomisin de kullanılmaktadır. Bu çalışmanın amacı, çeşitli klinik örneklerden izole edilen ve GSBL üreten E. coli izolatlarının temosilin, fosfomisin, ertapenem, imipenem, meropenem ve piperasilin-tazobaktam duyarlılıklarının olarak disk difüzyon yöntemiyle belirlenmesidir Gereç ve Yöntemler: Çalışmaya Ankara Yıldırım Beyazıt Üniversitesi Yenimahalle Eğitim ve Araştırma Hastanesi Mikrobiyoloji Laboratuvarı’nda çeşitli klinik örneklerden izole edilen ve genişlemiş spektrumlu beta laktamaz (GSBL) ürettiği çift disk sinerji yöntemiyle belirlenen 80 E. coli suşu dahil edildi. Suşların temosilin, fosfomisin, ertapenem, imipenem, meropenem ve piperasilin-tazobaktam duyarlılıkları disk difüzyon yöntemiyle CLSI önerileri doğrultusunda belirlendi. Bulgular: Çalışmaya dâhil edilen GSBL üreten E. coli suşlarında antibiyotik duyarlılık oranları sırasıyla ertapenem %100 (80/80), imipenem %100 (80/80) , meropenem %100 (80/80), temosilin %98,7 (79/80) , piperasilin-tazobaktam %96 (77/80) ve fosfomisin %95 (76/80) olarak belirlendi. GSBL üreten E. coli suşlarında karbapenemlere (ertapenem, imipenem ve meropenem) direnç saptanmadı. Sonuç: Hastanemizde GSBL üreten E. coli suşlarında temosilin ve fosfomisin duyarlılık oranlarının yüksek olduğu, temosilin ve fosfomisinin bu etkenlere bağlı enfeksiyonların tedavisinde karbapenemlere alternatif ilaçlar olarak kullanılabileceği belirlendi.
Purpose: Development of resistance to beta-lactam antibiotics with extended spectrum beta-lactam (GSBL) enzyme production E.coli and Klebsiella species( Klebsiella spp.) It is a mechanism of resistance. GBSL produced by E. coli and Klebsiella spp. In the treatment of the infections caused by themoline and phosphomycin have also been used in recent years. The aim of this study is to isolate E from various clinical samples and produce GSBL. Coli isolates are determined by the disque diffusion method as themilin, phosphomysine, ertapenem, imipenem, meropenem and piperasilin-tasobaktam sensitivities Tools and Methods: The study included 80 E. coli juice determined by the double disque synergy method produced by the extended spectrum beta-lactamaz (GSBL) isolated from various clinical samples in the Innovation and Research Hospital of Microbiology Laboratory. The sensitivities of souls; temoilin, phosphomycin, ertapenem, imipenem, meropenem and piperasilin-tasobaktam were determined in accordance with the CLSI recommendations by the disk diffusion method. Results: The GSBL producer involved in the work. The rates of antibiotic sensitivity in coli juices were set as: ertapenem 100% (80/80), imipenem 100% (80/80) , meropenem 100% (80/80), temosilin 98.7 (79/80) , piperasilin-tasobaktam 96% (77/80) and phosphomycin 95% (76/80) respectively. In the E. coli juices produced by GSBL, there was no resistance to carbapenems (ertapenem, imipenem and meropenem). The result: The high sensitivity rates of temoylin and phosphomycin in E. coli juices producing GSBL in our hospital have been determined that temoylin and phosphomycin can be used as alternative drugs to carbapenems in the treatment of infections associated with these factors.
Aim: Production of extended-spectrum beta-lactamase (ESBL) enzymes are the most common resistance mechanism against beta-lactam antibiotics on E. coli and Klebsiella spp. Temocillin and fosfomycin have also been used in the treatment of infections caused by ESBL producing E. coli and Klebsiella spp. The aim of this prospective study is to determine the susceptibility of temocillin, fosfomycin, ertapenem, imipenem, meropenem, and piperacillin-tazobactam to ESBL-producing E. coli isolate isolated from various clinical samples by the disk diffusion method. Material and Methods: The study included 80 E. coli strains isolated from various clinical samples in Ankara Yıldırım Beyazıt University Yenimahalle Training and Research Hospital, Microbiology Laboratory and ESBL producing were determined by double disc synergy method. The susceptibilities of the strains to temocillin, fosfomycin, ertapenem, imipenem, meropenem, and piperacillin-tazobactam were determined according to the CLSI recommendations by disk diffusion method. Determined by double disc synergy method. The susceptibilities of the strains to temocillin, fosfomycin, ertapenem, imipenem, meropenem, and piperacillin-tazobactam were determined according to the CLSI recommendations by disk diffusion method. Results: The antibiotic susceptibility rates of ESBL-producing E. coli strains included in the study were as follows; ertapenem 100% (80/80), imipenem 100% (80/80), meropenem 100% (80/80), temocillin 98.7% (79/80), piperacillin-tazobactam 96% (77/80) and fosfomycin 95% (76/80). Conclusion: Sensitivity of ESBL-producing E. coli strains against temocillin and fosfomycin were found to be high in our hospital. It has been determined that temocillin and fosfomycin can be used as alternative drugs to carbapenems in the treatment of infections due to these agents.
Alan : Sağlık Bilimleri
Dergi Türü : Uluslararası
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