Özet:

Abstract: In this research, the growth of Lactobacillus acidophilus bacteria in different medium of MRS Broth containing glucose, fructose, sucrose, 1-kestose, Orafti P95, fructose+1-kestose, fructose+nystose, sucrose+1-kestose, sucrose+nystose, glucose+1-kestose, glucose+nystose, or glucose+fructose+1-kestose was investigated. The results showed that Lactobacillus acidophilus bacteria can metabolize fructooligosaccharide mixtures, and preferentially uses 1-kestose. When nystose was combined with glucose, fructose or sucrose, no positive effect was observed on the growth. Thus, it was concluded that the bacteria cannot metabolize nystose. The predicted best triple combination of MRS-Broth containing glucose+fructose+1-kestose was inhibited the growth in comparison of double combinations. 1-kestose was found the best usable fructooligosaccharide compound whereas nystose was the least. Organic acid types and amounts that were produced after the consumption of fructo-oligosaccharides was analyzed by HPLC in Central Laboratory of METU, and it was found that 6.1 mg/mL lactic acid, 3.3 mg/mL propionic acid and 3.1 mg/ml butyric acid were produced by the bacteria.

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