Abstract:

Neuroblastoma (NB; SH-SY5Y cells) is important characteristic of the most widespread cancer in children after leukaemia and brain cancer. Discovery of new treatment approaches are required for NB disease. Epidemiological studies have demonstrated that different types of antioxidants are associated with decreased cancer and neurodegenerative diseases. Fruits and vegetables have a plenty source of Beta-carotene (BC), Alpha-tocopherol (vit E) and Ascorbic acid (vit C) that have potent free radicals scavenging actions and inhibitory properties to biochemical oxidation. BC, vit E and vit C increase the intracellular reactive oxygen species (ROS) generation and encourages cell apoptosis. It was found that these antioxidants resulted in an increase in the malondialdehyde (MDA) (marker of lipid peroxidation; LPO) and ROS levels at high concentration suggesting it behaves as a prooxidant in this study. The outcomes obviously indicated that studies of SH-SY5Y with dose-dependent BC, vit E and vit C results in oxidative damage through an increase in ROS and LPO generation and the expression of apoptotic marker caspase-3. These consequences find out additional evidences that these antioxidants could be involved in the process of apoptosis for new therapy in NB cells.

Keywords: