Özet:

Purpose: The purpose of this study is to investigate the expression of steroidogenic genes in the corpus luteum put in early pregnancy. In the animal model of the study, pregnant sheep are designed to be three subgroups; pregnancy 12: G12, pregnancy 16: G16 and pregnancy 22: G22. Day and Day 16 (S16). The expression level of steroidogenic genes (StAR, 3;HSD, P450scc) was assessed by quantitative polymerase chain reaction (PZR) and the localization of StAR mRNA was shown by the in situ hybridization method against the 16th day of pregnancy. Results: In pregnancy days (G16 and G22) StAR mRNA expression is higher compared to G12 (p<0.05) though there is no difference between G16 and G22 days (p>0.05). Similarly, the P450scc mRNA expression level was found more than G12 in pregnancy days in G22 and G16 (p<0.05). However, there is no difference in the expression of P450scc mRNA between G16 and G22 days (p>0.05). Similarly, the 3HSD mRNA expression level is greater than the G12 in pregnancy days in G22 and G16 (p<0.05). However, no change was detected between the days G16 and G22 in the 3rd HSD mRNA expression (p>0.05). From this study, in situ hybridization, S16 was not found in the luteal cells in the day. However, StAR mRNA was detected in luteal cells in G16 day. Recommendation: The level of expression of the genes found in the steroid pathway has been shown to play a critical role in early pregnancy in sheep. As a result, it may be recommended that the steroidogenic pathway in the corpus luteum of the sheep can transcribed the synthesis of progesterone necessary for the continuity of early pregnancy.

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