Özet:

In this study, to obtaine in vitro shoot regeneration in bitter vetch (Vicia ervilia L. Willd), shoot tip and cotyledon node explants were cultured on Murashige and Skoog (MS) medium containing different concentration of 6-benzylaminopurine (BAP; 0.5-2 mg/L), thidiazuron (TDZ; 0.25-1.5 mg/L) or meta-Topoline (mT; 1-4 mg/L) with 0.25 mg/L α-naphtalene acetic acid (NAA). Shoot regeneration frequency, the number of shoots per explant and shoot length were determined 7-8 weeks after the initiation of culture processes. The shoot regeneration frequency in the shoot tip explant was between 75-100% and between 57.14-92.85% in the cotyledon node explant. The maximum shoot numbers per explant were determined on medium containing 0.5 mg/L TDZ+0.25 mg/L NAA with 9.75 and 15.51 in both shoot tip and cotyledon node explants, respectively. While the highest shoot lengths were obtained on medium containing mT (1, 2 and 4 mg/L) at the shoot tips, it was measured on medium containing only 1 mg/L mT+025 mg/L NAA in the cotyledon node explants. MS medium containing 0.5, 1 or 2 mg/L indole-3-butyric acid (IBA) or half or full strength MS medium containing 1 mg/L IBA, 0-1 g/L activated carbon were used for rooting of regenerated shoots. Rooting varied between 0-9.713%.