Özet:

Molecular diagnosis for aspergillosis is less monitored due to the poor culture yield of Aspergillus spp. To estimate better this shortcoming, the Aspergillus spp. was detected and confirmed through respiratory samples from critically ill patients of Adewaniya city at the high risk of Aspergillosis. The study aimed to notice risk factors, clinical features, management, and outcomes in critically ill patients. PCR technology was used for the diagnosis of invasive Aspergillosis. For this purpose, initially,Magna pure LC instrument was used for automated extraction of fungal DNA of Aspergillus fumigatus and Aspergillus niger. Assay took 5-6 hours to perform. Oligonucleotide probes and primers were derived from the DNA sequence of 18S rRNA genes of varieties of fungal pathogens for species detection and identification. The database was used to assess fungal infection of Aspergillus fumigatus and Aspergillus nigerfrom samples of respiratory secretions of 100 (50% male, 50% female, between age 40-50) critically ill patients admitted in ICU.PCR assay gave high sensitivity and specificity for detection and identification of Aspergillus spp.from the fluid of respiratory tract of critically ill patients in the hospital ICU.

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