Özet:

This study was carried out to investigate the development of meristems obtained from tomato plants grown in vitro conditions in medium containing different concentrations of BAP (6-benzyl amino purine-cytokinin hormone) and IAA (İndole-3-acetic acid-auxin hormone). MS (Sigma Aldrich, Catalog No: M5519) medium was used as the main media and BAP and IAA were added separately at the rates of 0.1, 0.5 and 1.0 mg l-1 in this medium, and also cytokinin and auxin hormones were added together as concentrations of 0.5 mg l-1 BAP + 0.1 mg l-1 IAA and 0.1 mg l-1 BAP + 0.5 mg l-1 IAA in this medium in the research. As a result of the research, the highest success rate in terms of complete plant formation rate as 61.11%obtained from the medium containing 0.5 mg l-1 BAP + 0.1 mg l-1 IAA. The nutrient medium without any hormones gave the best results in terms of total direct regeneration (shoot, root and shoot + root) rate (100%), while the medium containing 0.5 mg l-1 BAP + 0.1 mg l-1 IAA gave the best result (100%) in terms of callus formation. MS medium containing 0.5 mg l-1 BAP + 0.1 mg l-1 IAA again showed the best result (340%) in terms of plant regeneration from the calluses. When the general result of the study was evaluated, it was determined that BAP or IAA hormones used in high concentrations encouraged callus formation in tomato meristems.

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