Özet:

Purpose: Recently, the use of five-value antimon compounds in the treatment of Leishmaniasis has been associated with the recurrence of the disease, drug resistance and serious side effects. Therefore, it is necessary to develop alternative therapy strategies. The aim of this study is to explore the laboratory effects of Zingiber officinale on the promastigots and amastigots of Leishmania major and Leishmania tropica. Methods: Leishmania major and Leishmania tropica promastigots and amastigots are produced collectively by cultivating in the RPMI1640 environment, enriched with other basic compounds. The MTT colorometric method and IC50 value have been used to evaluate the anti-Leishmania activity of the zencefiline hydroalcoholic extract. Results: Zingiber officinale's hydroalcoholic extract prevented the growth of Leishmania major and Leishmania tropica promastigots after 24, 48 and 72 hours of in vitro incubation. The IC50 of the hydroalcoholic extract of Zingiber officinale was detected at 56 μg/mL for Leishmania major after 72 hours and 275 μg/mL for Leishmania tropica promastigots. The IC50 of the hydroalcoholic extract of Zingiber officinale was 75 μg/mL for Leishmania major after 72 hours and 325 μg/mL for Leishmania tropica amastigotes. Results show that the hydroalcoholic extract of Zingiber officinale has cytotoxicity properties. Also, Leishmania tropica has a higher resistance than Leishmania major to the hydroalcoholic essence of Zingiber officinale. More research is needed on the subject.

Anahtar Kelimeler: