Özet:

In this study, it was aimed to determine the antibiotic susceptibility of Gram negative bacteria isolated from milk samples of cows with mastitis that were brought to Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Microbiology Laboratory between 2013 and 2018. A total of 1687 milk samples were cultured on blood agar and MacConkey agar. While 1708 microorganisms were isolated from 1352 milk samples, no any microorganisms were isolated from 335 samples. Isolation and identification of bacteria was made by conventional bacteriological methods and 151 (8.84%) of these bacteria were detected to be Gram negative. Escherichia coli, which constitutes 54.30% (82/151) of the Gram negative isolates, was the most isolated bacterium. Other Gram negatives were identified to be 17.88% Klebsiella pneumoniae, 7.95% Pseudomonas aeruginosa, 7.95% Citrobacter sp., 5.30% Pasteurella sp., 2.64% Enterobacter sp., 1.99% Proteus sp., 0.66% Flavobacter sp., 0.66% Alcaligenes faecalis and 0.66% Actinobacillus sp. In vitro resistance of these bacteria against to 9 antibiotics was determined by disk diffusion test. Over all 96.69% of the Gram negatives were found resistant to cloxacillin, 96.03% to erythromycin, 76.16% to oxytetracycline, 70.86% to amoxicillin, 54.97% to trimethoprim+sulfamethoxazole, 54.30% to gentamicin, 47.68 % to cefoperazone, 45.03% to amoxicillin+clavulanic acid and 29.80%, to enrofloxacin. All Gram-negative bacteria were resistant to at least two antibiotics, except for E. coli and Pasteurella sp. isolates. As a result, it was determined that E. coli was the most isolated Gram negative bacterium from bovine mastitis cases in Burdur province, followed by Klebsiella sp., Pseudomonas sp. and Citrobacter sp., due to the differences in antibiotic resistance profiles of Gram-negative bacteria, the treatment should be done according to antibiotic susceptibility test results.

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