Özet:

Purpose: increased oxygen stress plays an important role in the etiopathogenesis of chronic complications of diabetes. Our study aims to study the effects of vitamin C treatment in diabetic rats on oxidative and antioxidant events in the liver. The Wistar Albino rats were divided into 4 groups. 1.Control, 2.As-corbic acid (AA, Vitamin C) 3.Diabetes, 4.Diabetes+AA. 45 mg/kg Streptozotosin, solved in a group-diabetic citrate tampon, was administered in single dose, intra-peritoneal. After 48 hours, those with a hungry blood sugar above 200 mg/100 ml were taken to diabetes groups. AA and Diabetes+AA groups were controlled AA (20 mg/kg) soluble in mushroom water and Diabetes groups were administered in intragastric way, a single dose per day, for a period of 21 days. The animals were sacrificed under the thyopental sodium anesthesia. In the liver tissue, the levels of malondialdehitis, glutation and total nitric oxide (NOx) were measured. The differences were identified using the ANOVA and Mann-Whitney U tests. Results: According to control and AA groups, malondial-dehit was high, glutation and NOx were low in diabetes groups. When controlled, in the AA group, compared to Diabetes, malondialhydrate was low, glutation was high in the AA group. There is no significant difference between NOx values. In diabetics, vitamin C reduces the elevated levels of malondyroid in the liver, while it increases the levels of glutathion, adds foods rich in vitamin C to the diet or recommends the use of vitamin C preparations. Keywords: Diabetes, ascorbic acid, liver, lipid peroxides.   The Effects of ASCORBIC ACID ADMINISTRATION ON THE LIVER OXIDANT PROCESSES IN DIABETIC RATS Purpose: Increased oxidant stress plays an important role in the etiopatho-genesis of the chronic complications of diabetes. The aim of this study was to examine the effects of vitamin C treatment on the oxidant and antioxidant processes in the liver of diabetic rats. Materials and Methods: Wistar Albino rats were divided into 4 groups: 1-Control, 2-Ascorbic Acid (AA, Vitamin C), 3-Diabetes, 4-Diabetes+AA. For induction of diabetes, the rats were treated with a single dose of Strep-Tosotocin (45 mg/kg, i.p.). After 48 hours, rats whose fasting blood glucose levels were over 200 mg/100 ml were included in the diabetes groups. Rats in the AA and Diabetes+AA groups were treated with AA (20 mg/kg/day), which was administered intragastrically for 21 days. At the end of the ex-periment, malondialdehyde, glutathione and NOx levels in the liver tissues were determined. ANOVA and Mann-Whitney U tests were used for statis-tical analyses. Results: In the diabetes groups, malondialdehyde levels were increased, while glutathione and NOx levels were decreased. Malondialdehyde le-vels were decreased while glutathione levels were increased in the AA and Diabetes+AA groups in comparison with the controls. There were no significant-ficant differences among the groups in terms of NOx levels. Conclusion: The malondialdehyde and glutathione levels in vitamin C supplemented rat livers indicate that diets rich in vitamin C or vitamin C preparations might be beneficial in the treatment of diabetic patients. Key words: diabetes, ascorbic acid, liver, lipid peroxidation.