Abstract:

In this study, the effects of nicotine and melatonin were researched comparatively on the activities of superoxide dismutase (SOD) and catalase (CAT) as kidney antioxidants enzymes in mice. For this purpose, nicotine and melatonin were given to mice by intraperitonal injection and after the injection at the 2th, 4th, 8th, 12th and 24th hours mice were killed by servical dislocation; and their kidneys were removed. After total enzyme fractions were obtained by applying the kidneys homogenisation, sonification and centrifugation. These fractions were used for the determination of changes in SOD and CAT activities. As a result, it was determined that the CAT activity generally was inhibited but there was a few activation 8th hour by the effect of nicotine. Under the effect of melatonin the CAT activity generally was activated but there was a few inhibition in 2th hour. And it was observed that under the effect of nicotine+melatonin the CAT activity activated 4th and 8th hours but inhibited 2th , 12th and 24th hours. It was determined that the SOD activity was inhibited 2th and 4th hours but activated 8th, 12th and 24th hours by the effect of nicotine. Under the effect of melatonin the SOD activity generally was activated all the hours. And it was observed that under the effect of nicotine+melatonin the SOD activity inhibited 2th, 4th and 24th hours but activited 8th and 12th hours.

Keywords: