Özet:

Carbon tetracloride (CCl4) is an organic compound used in the production of refrigerant fluoroclorocarbons in the industry. CCl4 affects male reproductive functions negatively by damaging testicular cells. Curcetin is a well-known flavonoid with its antioxidant properties. In this study, we aimed at studying the impact of CCl4 on the damage caused by CCl4 on the testicles of male rats applied CCl4. In this study, a total of 28 adult Spraque-Dawley species male rats were used. The experimental animals were divided into a total of 4 groups, including Control Group, CCl4 Group, Circetin, CCl4 + Circetin Group. From this experimental animal; to the control group, the dose of olive oil once a day for 7 days, to the CCl4 group for 7 days is 0. 5 ml/kg CCl4 was administered in a single dose of 20 mg/kg per day for 7 days, and CCl4+ was administered in a single dose of 0.5 ml/kg CCl4 per day for 7 days, and in a single dose of 20 mg/kg per day for 7 days. The experimental animals were dropped with an anesthetic at the end of the experimental period and testis tissue samples were taken into the Bouin solution. Examples were blocked after routine histological processes. 3 μm cut from each block was painted with Hematoxylene-Eosine and Periodic Acid-Schiff + Hematoxylene paintings and microscopic examinations were carried out. According to the findings obtained in the study, normal histological structure in the testicles of the rats in the control group, intense spermogenetic cell damage in the testicles of the CCl4 applied rats, and reduced cell damage in the given group of CCl4 + curcetin. It has been found that CCl4 causes toxicity on the testicles of male rats, while the circle reduces this damage that CCl4 causes in the testicles. Keywords: carbon tetrachloride, curcetin, rats, testis Abstract: Carbon tetrachloride (CCl4), the industry refrigerant is an organic compound used in the production of chlorofluorocarbon. Damage to testicular cells by CCl4 adversely affect male reproductive functions. Quercetin is a flavonoid antioxidant properties are well known. In this study, the testes of male rats on applied CCl4 damage caused by CCl4 aimed to investigate the effect of quercetin. In this study, a total of 28 adult male Spraque Dawley rats were used. Laboratory animals; Control group, CCl4 group, quercetin group, including CCl4 + quercetin group was divided into 4 groups. This experimental animals; control group for 7 days, once daily determined dose of olive oil, CCl4 group daily for 7 days of a 0.5 ml/kg CCl4, quercetin group daily for 7 days in a single dose of 20 mg/kg of quercetin, CCl4 + quercetin group for 7 days once a day 0.5 ml/kg daily for 7 days CCl4 20 mg/kg were injected with quercetin. Experimental animals were anesthetized with anesthetic agent at the end of the test period, and testicular tissue samples taken up in Bouin solution. After the staining process was blocked. 3 mm sections taken from each block hematoxylin and eosin and periodic acid-Schiff + were stained with hematoxylin and microscopic examinations were performed. According to the findings obtained from the study, the normal histological structure of the testicles of rats in the control group, intense spermogenic cells in the testicles of rats by CCl4 injury, CCl4 + quercetin decreased cell damage was observed in the group. As a result, the testicles of male rats on CCl4-induced toxicity, quercetin is formed in the testicles of CCl4 was found to reduce this damage. Keywords: carbon tetrachloride, quercetin, rat, testes

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