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Bu çalışmanın amacı, Aksaray Malaklı çoban köpeği spermasının farklı katkı maddeleri içeren sulandırıcılar ile dondurulup çözüm sonu spermatolojik parametrelerin araştırılmasıdır. Çalışma grupları, Tris ana stok (TAS)+trehaloz (10 mM), TAS+metiyonin (2 mM) ve kontrol olarak oluşturuldu. Köpeklerden sperma digital maniplasyon yöntemi ile alındı. Sulandırma işlemi tamamlanan sperma örnekleri 0.25 ml lik payetlere çekildi. Payetlenen spermalar 1.5 saat +5˚C’ta ekilibrasyona tabi tutuldu. Ekilibrasyon sonrası payetler sıvı azot buharında donduruldu ve sıvı azot içerisinde saklandı. Sperma hacminin belirlenmesinde dereceli sperma toplama tüpleri kullanıldı ve sonuçları mililitre olarak kaydedildi. Hemositometrik yöntem ile spermatozoon konsantrasyonu belirlenerek mm3 olarak kaydedildi. Ölü canlı spermatozoa muayenesi için eosin-nigrosin boyama yöntemi kullanıldı. Spermatozoa plazma membran bütünlüğü oranını belirlemek için Hipo osmotik şişirme (HOS) testi yapıldı. Sonuçlar istatistiksel açıdan tek yönlü varyans analizi ile incelendiğinde çözüm sonu metiyonin grubunda motilite (%52,5±2,7) ve HOS test parametreleri açısından (%43,1±2,9), kontrol grubuna (%41,5±3,1;%33,2±2,9) göre istatistiksel farklılık gözlendi (p<0,05). Çözüm sonu canlı spermatozoa oranına en yüksek metiyonin grubunda ulaşılmasına rağmen gruplar arası istatistiksel farklılık bulunamadı (p>0,05). Trehaloz grubunda ise motilite, HOS test ve canlı spermatozoa oranı sırasıyla %50,0±3,9;%60,6±3,7;%38,4±3,6 olarak bulundu. Çalışmanın sonucunda Aksaray Malaklı çoban köpeği spermasında tris bazlı sulandırıcıya eklenen metiyonin 2mM dozunun spermatolojik parametreler üzerine faydalı olduğu belirlenmiştir. Metiyoninin kriyoprotektif etkinliğinin trehaloz ve kontrol grubuna göre daha güçlü olduğu kanısına varıldı.
The purpose of this study is to investigate the spermatological parameters of the sperm of Aksaray Malaklı Shepherd Dog with hydrators containing different additives and the solution ends. The study groups were created as Tris main stock (TAS)+trehaloz (10 mM), TAS+metionine (2 mM) and control. Sperm is taken from dogs through digital manipulation method. Sperm samples that were completed with the hydration process were drawn into 0.25 ml shells. The split sperm was submitted to ecilibration at 1.5 hours +5 ̊C. After the ecillibration, the shells were frozen in a liquid nitrogen steam and stored in the liquid nitrogen. To determine the volume of sperm, scale sperm collection pipes were used and the results were recorded in milliliters. The hemosithometric method determined the sperm concentration and was recorded as mm3. The eosin-nigrosin coloring method was used for the examination of the dead living sperm. Spermatozoa plasma membrane integrity ratio was tested for Hipo osmotic swelling (HOS). When the results were studied by one-way variance analysis from a statistical point of view, a statistical difference was observed in terms of motility (%52,5±2,7) and HOS test parameters (%43,1±2,9), in terms of control group (%41,5±3,1;%33,2±2,9) in the solution end group of methionine (p<0,05). Although the solution ended at the highest level of living sperm in the group of methionins, there was no statistical difference between the groups (p>0,05). In the Trehaloz group, the rate of motility, HOS test and living sperm was 50,0 ± 3,9; 60,6 ± 3,7; 38,4 ± 3,6 respectively. According to the study, the dose of methionine 2mM added to the tris-based moisturizer in the sperm of Aksaray Malaklı shepherd dog has been determined to be beneficial on the sperm parameters. It is believed that the cryoprotective effect of methionin is stronger than the trehaloz and control group.
The aim of this study was to investigate the spermatological parameters of Aksaray Malakli shepherd dog after freeze-thawing semen with various additives. Study groups were set up as Tris-based extender (TBE)+trehalose (10mM), TBE+methionine (2mM) andcontrol. Sperm was collected by bulbus glandis massage (Digital Manipulation). Diluted semen samples were aspirated into 0,25 ml French straws and equilibrated at 5 ºC for 1,5 h. After equilibration, the straws were frozen in liquid nitrogen vapor, plunged into liquid nitrogen for storage. For determination the sperm volume, grade semen collecting tubes were used and the results were recorded in milliliters. The sperm concentration was determined with hemocytometric method as mm3. Eosin-nigrosine staining method was used for live sperm percentages. The HOS-test was performed to determine the ratio of intact spermatozoa membrane. When the freeze-thawing results are statistically examined with one-way analysis of variance, methionine group, motility (52,5%±2,7) and HOS test analysis (43,1%±2,9), (41,5%±3,1;33,2%±2,9) shown significant differences compared control (41,5%±3,1; 33,2%±2,9) group (p <0,05). The best result in the live sperm percentages was observed in methionine group but there was no statistically significant difference between the groups (p> 0.05).In the trehalose group, motility, HOS test and live sperm percentages results were 50.0% ±3,9;60,6%±3,7;38,4±3,6%, respectively. As a result of the study, it was determined that methionine 2 mM added to tris-based diluent in Aksaray Malaklı shepherd dog sperm was beneficial on spermatological parameters. The cryoprotective effect of methionine was found to be stronger than trehalose and control group.
Alan : Sağlık Bilimleri
Dergi Türü : Ulusal
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