Özet:

The goal in the cancer treatment is to induce tumor cell death without damaging healthy cells and to prevent the spread of these cells to other organs. In the case of metastasis, the cure protocols may be inadequate. Alternative methods are used to support treatment. For this purpose, herbal products are widely used. In our experiment, we aimed to investigate the effect of Hypericum crenulatum polysaccharides on the proliferation of breast cancer cell lines. Hypericum crenulatum polysaccharides were isolated and were exposed to the MCF-7 and MDA-MB-231 cells  at different dilutions,  then IC50 doses for each cells were calculated using MTT assay. Breast cancer cells were treated with IC50 doses of polysaccharides for 24 hours and immunocytochemistry was performed to evaluate the expressions of metastases and proliferation markers, PI3K, Akt-1 and Erk-1/2. The staining results were evaluated by H-score and the difference between the groups was analyzed statistically. Hypericum crenulatum polysaccharides were found to be toxic to both breast cancer cells by MTT method. When PI3K, Akt-1 and Erk-1/2 markers of proliferation and metastasis were evaluated, it was seen that there was a significant decrease compared to the control group. Since MDA-MB-231 cells are ER (-), their invasion ability is over compared to MCF-7 ER (+)  cells. For this reason, the toxic effect of polysaccharides was found to be higher in MCF-7 cells. As a result, in vivo studies are needed to be performed following the the antiproliferative effects of herbal products are found in vitro.

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