Özet:

ABSTRACT Background: In this study, it was aimed to determine the expression levels of tumor suppressor microRNAs (miRNA) including miR-143, miR-145 and miR-192 in tumor and normal colon / rectum tissues of Stage II colorectal cancer (CRC) patients by using qPCR and to compare the obtained data. Materials and Methods: This study was performed on tumor or normal (control-clean surgical margin) colon / rectum tissues of 9 patients diagnosed with colorectal cancer as a result of clinical evaluation with laboratory and pathological findings in Gaziantep University Medical Faculty Research and Practice Hospital General Surgery Department. miRNAs isolated with miRNeasy mini kit were used for qPCR. Isolated miRNAs were converted into cDNA by miScript RTPCR commercial kit. The miRNA expression levels of tissue samples were determined by using RT- SYBR Green qPCR kit in Rotor Gene Q. Result: The mean level of miRNA-143 expression in 9 patients diagnosed with colorectal cancer appears to have decreased 0.096 fold compared to normal tissue and tumor tissue, while miR-145, 1.52 fold. This decrease was considered as statistically significant (p <0.05). When the fold chance values of normal and tumor tissue are compared, despite being tumor supressor miRNA, miR-192 appears to have increased 3.25 fold and was not considered statistically significant (p>0.05). Conclusion: According to the findings obtained in our study, the expression levels of tumor supressor miRNA miR-143 and miR-145 in tumor colon tissues of patients with colorectal cancer have decreased. miRNAs have attracted major interest as a means to analyze the molecular pathways involved in cancer development and progression. In addition to their important cellular functions, it is possible that secreted miRNAs may be diagnostic biomarkers for cancer detection. 

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