Özet:

Recent studies have shown that adipose tissue is not only an energy store, but also an endocrine organ. Obesity, which occurs as a result of increased fat mass in the body, is observed together with increased oxidative stress and low-grade chronic inflammation. It is an increase in tissue oxidative stress due to the inflammation observed in adipose tissue. Antioxidant enzymes are proteins that help suppress the severity of oxidative stress by leading to the formation of fewer active radicals or reducing the damage of a free radical chain reaction on proteins, lipids, carbohydrates, and DNA. Catalase (CAT), an important antioxidant enzyme, prevents the formation of oxidative stress by breaking down H2O2 into water and oxygen. The fact that adipose tissue has a high lipid and low protein content, high lipid interference, makes protein isolation and activity measurements in this tissue difficult. In our study, it was aimed to investigate the effect of using different protein isolation methods on CAT activity in adipose tissue and to identify the necessary conditions for measurement. CAT activity measurements were performed using the Aebi method. Retroperitoneal adipose tissue was removed from the rats and activity measurements were performed using three different homogenization methods. Chloroform/methanol was used as the organic solvent in homogenization 1 (H1), in homogenization 2 (H2) and homogenization 3 (H3) only chloroform was used . As a result of the evaluations, the average specific activity values of the H1 method were found to be higher than those of the other two methods. As a result, it was concluded that the H1 method may be more suitable for measuring CAT enzyme activity in retroperitoneal adipose tissue.

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