Özet:

The purpose of this study is to investigate the effects of the solution of the different doses of crocidine added to the coach’s sperm irrigator on the spermatological parameters, oxidative stress and DNA damage. The ejaculates were gathered with the help of the artificial vagina once a week by the coach of five heads of Merinos, and this process was repeated six times. The ejaculates were divided into four sections with non-antioxidant (control) and antioxidant (0.5 mM, 1 mM and 2 mM) moisturizers that will be 150x106 sperm in ml. The hydrated samples were frozen in a liquid nitrogen steam after 3 hours of ecilibration at 0C at 0.25 mL in the shells. The solution ended with the highest values in 0.5 mM and 1 mM groups according to the control group in terms of subjective motility (P<0.05). In addition, an abnormal sperm rate has been significantly reduced (P<0.05) in the group containing 1 mM of chrosin according to the control group. The highest values were obtained in the 1 mM group (P<0.05) according to the control group in the Host-Eozin test. In terms of DNA damage, reductions in groups containing 0.5 mM and 1 mM chrosine were statistically significant (P<0.05) according to control group. The total oxygen status was significantly reduced in all antioxidant groups (P<0.05). As a result, the study found that the 1 mM group of different doses of chrosin involved in the sperm in the storage of coach sperm by freezing provided the best protection in terms of sperm motility, abnormal sperm ratio, HOST-Eosin test ratio, oxidative stress and DNA damage control group and other groups.

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