Özet:

This study was performed to determine the monocyte nitric oxide (NO) levels in naturally infected cattle with Theileria annulata (T.annulata) in Yeşilhisar and İncesu districts of Kayseri where the disease is enzootic. This is the first field study investigate the NO levels in monocytes of cattle naturally infected with T. annulata in Turkey. Blood samples and ticks were collected from the animals which were suspected of theileriosis. Blood samples were also collected from the cattle on Erciyes University Farm as negative control. Theileria annulata infection was determined by microscopical examination (ME), Indirect Fluorescent Antibody Technique (IFAT) and molecular (PCR, RLB) examinations. Ticks collected from the animals were identified and examined for the T. annulata infection. Leukocytes were isolated by buffy-coat method and nitrite levels as indicator of NO were determined with a colorimetric method using Griess reagent. The prevalence of T. annulata was 53.33% by ME, 60.00% by IFAT and 50.00% by RLB, and no statistically significant difference was found among these methods (p>0.05). Adult ticks were observed in 31.6% of the animals included in the study, and they were identified as Hyalomma anatolicum anatolicum, H. m. marginatum, H. d. detritum, H. a. excavatum. Only 20% of H. a. anatolicum were found to be naturally infected with T. annulata. The NO levels in animals with acutely and severely infected were significantly higher (p<0.001) than the subclinically infected and control animals. When packet cell volume (PCV) levels were determined in uninfected, subclinically infected, acutely infected, severely infected (agony) and control animals, The PCV in acutely and severely infected animals were significantly lower (p<0.001) than the subclinically infected and control animals. Furthermore, with respect to PCV, the difference between acutely and severely infected animals was also significant (p<0.001). The results of this study to ınvestigate that infected macrophages with T. annulata result in the induction of NO production compared to uninfected cells

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