Abstract:

Blastocystis species are among the gastrointestinal system parasites that are common in developing countries today. In the diagnosis, microscopic methods, such as native-lugol and trichrom paint, are mostly used, and cultural and molecular methods are preferred for research purposes. In this study; Blastocystis spp in children between 0-12 years of age with acute diarrhea in the Eskişehir province. The success and success of the methods used in the diagnosis are compared. Examples of ejaculation taken from children who apply to the emergency service of two different hospitals with acute diarrhea and from children who go to primary school at seven different socio-economic levels with no diarrhea were included in the study. Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples Examples The study was approved when the ethics of Eskişehir Osmangazi University was established and was supported by the Eskişehir Osmangazi University Research Projects. A total of 303 output samples were included in the study, 84 of which were taken from children with acute diarrhea. Blastocystis spp. The observation rates were determined by the direct microscopy method by 38%, by the Tricrom coloration method by 35% and by the molecular method by 38%. In 84 cases with acute diarrhea complaints, these rates were identified as 13%, 18% and 18% respectively. When compared methods with reference to the PCR method in the acute diarrhea group, the sensitivity of the direct microscopic method was 65%, the specificity of the trichromic method was 78%, the sensitivity of the trichromic method was 55%, the specificity of the trichromic method was 77%, the sensitivity of the PCR method was 80%, the specificity of the trichromic method was 84%. As a result, the sensitivity and peculiarity of molecular methods can be preferred for more reliable results in diarrhoea cases, but there is a need for a multi-centric and comprehensive clinical study.

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