Roberts’ False Brook Salamander (Pseudoeurycea robertsi) is a critically endangered plethodontid salamander, endemic to the Nevado de Toluca Volcano (NTV), Mexico. Little is known about the biology and ecology of this species, including its microhabitats. Thus, this study aimed to collect basic information about P. robertsi. We sampled fourteen forested sites in the NTV; to corroborate the correct identification of the species we used genetic data, we assessed the variation in head morphometric measurements and dorsal colouration patterns amongst localities and the microhabitat features associated with P. robertsi presence. Of the four potential salamander species, P. robertsi was the most abundant (89.80%) and widely distributed (approximately within 130 km2) salamander in the NTV. We did not find significant variations in morphometry; however, we found significant differences in dorsal patterns between populations (in the number and size of segments of the dorsal stripe). The average total length for 185 adults was 89.15 mm (38.7–117.9 mm); we found seven patterns of dorsal stripe. We found 98% of P. robertsi individuals under the bark of fallen logs in Abies religiosa and A. religiosa-Pinus sp. forests, with a higher number of detected salamanders in naturally-fallen logs than in cut logs (34% vs. 10%). Thus, keeping well-preserved A. religiosa forests and retaining fallen logs is essential to P. robertsi conservation.
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