Özet:

Haloalkalitolerant fungus can live in environments where the salt concentration and pH values are high. Bioactive compounds produced under stressful conditions have potential biotechnology applications. In this study, 52 microfoons isolated from Acıgöl/Turkey with polyester conditions have been scanned in terms of some biotechnological characteristics. Antimicrobial and antioxidant activities have been determined by the use of agar diffusion and 2,2-difenyl-1-pikrilhydrazil (DPPH) radical disinfectant method, respectively. The enzyme activity is determined by various methods in which the agar diffusion technique is used. Silver nanoparticles (AgNP) synthesis is performed using a microfungus filter that does not contain the cell. 40 percent of the isolates showed antimicrobial activity against at least one of the test microorganisms. Penicillium dipodomyicola showed the highest antibacterial activity against E. coli and S. aureus, while P. brevicompactum showed the highest activity against Candida albicans. Penicillium dipodomyicola and P. bilaiae have a level (90% and above) of free-radicals polishing activity that can compete with positive control. All the isolat with amylase activity belong to the species Aspergillus and Penicillium, and are identified as the highest activity species of A. ochraceous, A. flavus and P. brevicompactum. 55% of the isolates show proteolithic activity, while A. alliaceus was the highest-activity isolate. Almost all isolates (92%) showed lipolithic activity. In particular, the A. amstelodium, P. sizovae and P. solitum islands have a significant level of lipolithic activity. 35% of isolates showed selulolithic activity and high activity was observed in Cladosporium pseudocladosporioides, P. dipodomyicola and P. bilaiae isolates. Eight of the isolates performed AgNP synthesis within 24 hours. When all the results were evaluated, it was determined that insulates such as Aspergillus amstelodami, A. ochraceus, Penicillium dipodomyicola and P. brevicompactum have the potential to serve in different industrial fields.

Anahtar Kelimeler:

Özet:

Haloalkalitolerant fungi can survive in environments with high salt concentrations and pH values. The bioactive compounds produced under stressful conditions have potential biotechnological applications. In this study, 52 microfungi isolated from Acıgöl Lake in Türkiye, offering polyextreme conditions, were screened for some biotechnological properties. The antimicrobial and antioxidant activities of the isolates were determined using the agar diffusion and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging methods, respectively. Enzyme activities were determined by various methods using the agar diffusion technique. Synthesis of silver nanoparticles (AgNPs) was carried out using cell-free filtrate of microfungi. 40% of the isolates showed antimicrobial activity against at least one of Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213 and Candida albicans ATCC 90028 used as the test microorganisms. Penicillium dipodomyicola showed the highest antibacterial activity against E. coli and S. aureus, while P. brevicompactum showed the highest activity against C. albicans. Penicillium dipodomyicola and P. bilaiae were found to have free radical scavenging activity of a level (90% and above) that can compete with positive control. All of the isolates with amylase activity belonged to Aspergillus and Penicillium and the most prominent three of them were A. ochraceous, A. flavus and P. brevicompactum. 55% of the isolates showed proteolytic activity, among which A. alliaceus had the highest activity. Almost all isolates (92%) showed lipolytic activity. Aspergillus amstelodami, P. sizovae and P. solitum had a significant level of lipolytic activity. 35% of the isolates showed cellulolytic activity with highest values Cladosporium pseudocladosporioides, P. dipodomyicola and P. bilaiae. Eight of the isolates carried out AgNP synthesis within 24 h. When all the results were evaluated, Aspergillus amstelodami, A. ochraceus, Penicillium dipodomyicola, and P. brevicompactum appeared to have the potential to serve in different industrial areas.