Abstract:

Medicinal properties of Vincetoxicum genus have been known for a long time. Leaves, dry seeds, and rhizomes of some Vincetoxicum species have been used in folk medicine for the treatment of injuries, scabies, neurosis, scrofula, malaria, wound, fever, rupture, and external cancers. The present study focused on evaluating anti(myco)bacterial, antifungal, and antiproliferative activities of seeds of V. canescens subsp. canescens and V. cancescens subsp. The endemic pedunculata. Ethanol and hexane extracts of seeds of two plants were tested against three fungal strains (Candida albicans, Candida tropicalis, and Candida glabrata), two Gram-positive bacterial strains (Bacillus subtilis and Staphylococcus aureus), three Gram-negative bacterial strains (Aeromonas hydrophila, Escherichia coli, and Acinetobacter baumannii), and Mycobacterium tuberculosis H37Rv using broth microdilution method. Fluconazole, Ethambutol, Ampicillin, and Isoniazid were used as reference drugs. Antiproliferative effects of various concentrations (200, 100, 50, and 25 μg/mL) of ethanol and hexane extracts were tested against A549 human lung cancer cell lines using MTT test. The ethanol extracts were found to be more effective against A. baumannii (62. 5 μg/mL MIC value) than Ampicillin (125 μg/mL MIC value). Against A549 human lung cancer cell lines, 100 μg/mL concentration of ethanol extract of V. canescens subsp. canescens seeds and 200 μg/mL concentration of ethanol extract of V. cancescens subsp. Pedunculata seeds were exhibited statistically lower cell viability levels than control groups (P <0.05). As a result, V. canescens subsp. canescens and V. cancescens subsp. pedunculata seeds showed antimicrobial and antiproliferative effects against all tested microorganisms and A549 human lung cancer cell lines; moreover, ethanol extracts were found to be more effective than hexane extracts.

Keywords:

Abstract:

Medicinal properties of Vincetoxicum genus have been known for a long time. Leaves, dry seeds, and rhizomes of some Vincetoxicum species have been used in folk medicine for the treatment of injuries, scabies, neurosis, scrofula, malaria, wound, fever, rupture, and external cancers. The present study focused on evaluating anti(myco)bacterial, antifungal, and antiproliferative activities of seeds of V. canescens subsp. canescens and V. cancescens subsp. pedunculata (endemic). Ethanol and hexane extracts of seeds of two plants were tested against three fungal strains (Candida albicans, Candida tropicalis, and Candida glabrata), two Gram-positive bacterial strains (Bacillus subtilis and Staphylococcus aureus), three Gram-negative bacterial strains (Aeromonas hydrophila, Escherichia coli, and Acinetobacter baumannii), and Mycobacterium tuberculosis H37Rv using broth microdilution method. Fluconazole, Ethambutol, Ampicillin, and Isoniazid were used as reference drugs. Antiproliferative effects of various concentrations (200, 100, 50, and 25 μg/mL) of ethanol and hexane extracts were tested against A549 human lung cancer cell lines using MTT test. The ethanol extracts were found to be more effective against A. baumannii (62.5 µg/mL MIC value) than Ampicillin (125 µg/mL MIC value). Against A549 human lung cancer cell lines, 100 µg/mL concentration of ethanol extract of V. canescens subsp. canescens seeds and 200 µg/mL concentration of ethanol extract of V. cancescens subsp. pedunculata seeds were exhibited statistically lower cell viability levels than control groups (P <0.05). As a result, V. canescens subsp. canescens and V. cancescens subsp. pedunculata seeds showed antimicrobial and antiproliferative effects against all tested microorganisms and A549 human lung cancer cell lines; moreover, ethanol extracts were found to be more effective than hexane extracts.