Antimikrobiyal ajan olarak kullanılan ilaçların olası yan etkileri ve mikroorganizmaların bu ilaçlara karşı direnç kazanması, insan sağlığı için büyük tehlike oluşturmaktadır. Bu durum bilim çevrelerini yeni arayışlara itmiştir. Gıda olarak tüketilen pek çok bitkisel ürünün aynı zamanda hastalıklardan korunma amacıyla da halk tarafından kullanıldığı bilinmektedir. Ancak, bunlardan pek çoğu halen bilimsel verilerle doğrulanmamıştır. Bu bakımdan, mevcut çalışmada Elazığ ilinden toplanan ve yöre halkı tarafından geleneksel olarak pek çok rahatsızlığın hafifletilmesinde kullanılan Morus nigra L. (karadut) taze meyve ekstraktlarının (etanol, metanol ve etil asetat) pomolojik özellikleri, toplam fenolik ve toplam flavonoid içerik değerleri ile toplam antioksidan kapasiteleri belirlendi. Bununla birlikte, etanol ekstraktının fitokimyasal içeriği RP-HPLC ile değerlendirildi. Ayrıca bunların, antimikrobiyal özellikleri ve antibiyotiklerle (kloramfenikol, novobiosin ve nalidiksik asit) olan sinerjileri test edildi. Etanol ekstraktının toplam fenolik içerik değeri ve antioksidan özellikleri diğerlerine göre anlamlı derecede yüksek bulundu. Bu ekstraktın major bileşeni ise klorojenik asit olarak tespit edildi. Ekstraktların test mikroorganizmalarına karşı düşük etkinlik gösterdiği, bununla beraber metanol ekstraktı/kloramfenikol kombinasiyonu haricinde tüm ekstrakt antibiyotik karışımlarının ciddi bir patojen olan P. aeroginosa üzerine tam sinerjik etki gösterdiği tespit edildi. Tam sinerji olması ise, antibiyotiğin daha düşük dozda daha etkili olmasını sağlaması açısından oldukça önemlidir. Sonuç olarak, M. nigra meyve ekstraktlarının sahip olduğu biyolojik aktiviteleri nedeniyle, gıda ve ilaç endüstrilerinde doğal ve alternatif bir kaynak olarak kullanılma potansiyeline sahip olduğu düşünülmektedir.
The possible side effects of drugs used as an antimicrobial agent and the gain of resistance of microorganisms to these drugs pose a great danger to human health. This has pushed the scientific environments to new searches. Many of the vegetable products consumed as food are also known to be used by the public for the purpose of protecting against diseases. However, many of them have not yet been confirmed by scientific data. In this regard, the current study, which was collected from the Elazığ province and traditionally used by the local people in the relief of many disorders, determined the pomological characteristics of Morus nigra L. (caradut) fresh fruit extract (etanol, methanol and ethyl acetate), the total phenolic and total flavonoid content values and the total antioxidant capacity. However, the phytochemical content of the ethanol extract was assessed by RP-HPLC. They also tested their anti-microbial properties and synergies with antibiotics (chloramfenicole, novobiosine and nalidic acid). The total phenolic content value and antioxidant properties of ethanol extract were found significantly higher than others. The main component of this extract was identified as chlorogenic acid. It was found that the extract was low-effective against the test microorganisms, and that all the antibiotic compounds of the extract except the methanol extract/chloramfenicol combination had a full synergistic effect on a serious pathogen P. aeroginosa. To be fully synergistic, it is very important to ensure that the antibiotics are more effective in lower doses. As a result, M. nigra fruit extract is believed to have the potential to be used as a natural and alternative source in the food and pharmaceutical industries due to its biological activity.
Possible side effects of drugs used as antimicrobial agents and microorganisms' resistance to these drugs pose a great danger to human health. This situation led to the scientific community to new searches. It is known that many herbal products consumed as food are also used by the public for the protection of diseases. However, many of these are still not confirmed by scientific data. In this regard, in the current study, Morus nigra L. (black mulberry) fresh fruit extracts (ethanol, methanol and ethyl acetate) collected from Elazığ province and used by local people to alleviate many diseases, total antioxidant content of total phenolic and total flavonoids. capacities were determined. However, the phytochemical content of the ethanol extract was evaluated by RP-HPLC. In addition, their antimicrobial properties and synergies with antibiotics (chloramphenicol, novobiosin and nalidixic acid) were tested. The total phenolic content value and antioxidant properties of ethanol extract were significantly higher than the others. The major component of this extract was determined as chlorogenic acid. It was determined that the extracts showed low efficacy against the test microorganisms, however, all extract antibiotic mixtures exhibited a full synergistic effect on P. aeroginosa, a serious pathogen, except for the methanol extract / chloramphenicol combination. The full synergy is important in terms of ensuring that the antibiotic is more effective at a lower dose. As a result, due to the biological activities of M. nigra fruit extracts, it is thought to have the potential to be used as a natural and alternative source in the food and pharmaceutical industries.
Alan : Fen Bilimleri ve Matematik; Mimarlık, Planlama ve Tasarım; Mühendislik; Ziraat, Orman ve Su Ürünleri
Dergi Türü : Uluslararası
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