Abstract:

As the name suggest, real-time PCR is a technique used to monitor the progress of a PCR reaction in real-time.Quantitative real-time-polymerase chain reaction (RT-PCR) technology is many alternative instruments and fluorescentprobe systems have been developed recently. The technique of real-time PCR has features that make its useadvantageous in a wide range of applications. The development of fluorescent methods for a closed tube polymerasechain reaction has greatly simplified the process of nucleic acid quantification. Real-time PCR applications include:quantitative mRNA expression studies, DNA copy number measurements in genomic or viral DNAs, allelicdiscrimination assays or SNP genotyping, drug therapy efficacy, DNA damage measurement.

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