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  Citation Number 1
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Koç spermasının ekilibrasyon ve dondurma-çözdürme sonrası spermatolojik ve biyokimyasal parametreleri üzerine lipoik asitin etkisi
2011
Journal:  
Eurasian Journal of Veterinary Sciences
Author:  
Abstract:

Amaç: Koç sperması sulandırıcısına katılan farklı dozlardaki lipoik asitin spermanın ekilibrasyon ve dondurma sonrası bazı spermatolojikve biyokimyasal parametreler üzerine etkilerinin araştırılması amaçlandı. Gereç ve Yöntem: Araştırmada 6 baş ergin Konya Merinosu koçlara ait ejakülatlar kullanıldı. Ejakülatlar 4 eşit hacme bölünerek lipoik asit 1, 2, 4 mM içeren ve içermeyen (kontrol) Tris sulandırıcısıyla mL'de yaklaşık 400 x 106 spermatozoa olacak şekilde 37 ºC'ta sulandırıldı ve donduruldu. Bulgular: Ekilibrasyon sonrası lipoik asitin kontrol gruplarına göre sperm parametrelerinde etkisi görülmezken, dondurma-çözdürme sonrası numunelerde lipoik asit 1 mM (%74.3), kontrol (%62.5) ve lipoik asit 4 mM (%65.0) gruplarına göre motilite yönünden önemli derecede iyileştirme sağladı (p<0.001). Spermatozoanın HOS test ve ak-rozom bütünlüğü üzerinde dondurma-çözdürme sonrası lipoik asitin etkinliği görülmedi. Lipoik asitin 1, 2 ve 4 mM dozlarının ekilibrasyon ve dondurma sonrası biyokimyasal parametreler yönünden kontrol gruplarına göre etkinlikleri önemli bulunmadı (p>0.05). Öneri: Koç spermasına 1mM dozda lipoik asit ilavesi dondurma-çözdürme sonrası motiliteyi arttırmaktadır. Bununla birlikte lipoik asitin farklı sulandırıcılarda ve hayvan türleri-ırklarında doz denemelerinin yapılması gerekmektedir.

Keywords:

The effect of lipoic acid on the spermatological and biochemical parameters after the ecilibration and ice-solving of the coch sperm
2011
Author:  
Abstract:

Purpose: The study of the effects of different doses of lipoic acid involved in the coch sperm irrigator on some spermatological and biochemical parameters on the ecilibration and after ice cream sperm. Method and method: In the study, 6 main adult Konya Merinosu coaches used ejaculates. The ejaculates were divided into 4 equal volumes, containing 1, 2, 4 mM of lipoic acid and not containing (controlled) Tris hydratant, so that it would be about 400 x 106 spermatozoa in mL and frozen at 37 oC. Results: After Ekilibration lipoic acid has no effect on the sperm parameters according to the control groups, while after ice-solving samples have significantly improved the motility according to the control groups of 1 mM of lipoic acid (%74.3), 62.5) and 4 mM of lipoic acid (%65.0). by 001 . The effect of lipoic acid after ice-solving on the HOS test and ac-rozom integrity of the sperm was not observed. The effects of the 1, 2 and 4 mM doses of lipoic acid were not significant according to the control groups in terms of equilibration and biochemical parameters after ice cream (p>0.05). Recommendation: A dose of 1mM of lipoic acid in the coch sperm increases the motility after ice-solving. However, doses of lipoic acid should be tested in different moisturizers and animal species.

Keywords:

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Eurasian Journal of Veterinary Sciences

Field :   Sağlık Bilimleri

Journal Type :   Uluslararası

Metrics
Article : 1.407
Cite : 1.280
2023 Impact : 0.068
Eurasian Journal of Veterinary Sciences