Özet:

We investigated the wound healing efficacy of the Prunus Spinosa L. using an excisional cutaneous wound model in mice. For this purpose methanol extracts of fruits prepared. We used 32 number C57/Black mice in total; equally divided into four groups. Group 1 control, group 2 vechile (Glycerine), group 3 Prunus Spinosa L., group 4 madecassol. Prunus Spinosa L.and madecassol were applied for 10 days by creating a excisional wound model on the back of each mouse in each group. At the end of the 10th day, wound areas were taken for histologic follow-up, scoring with hematoxylen-eosin and immunohistochemical (TGF-β, COL1A1) staining. Wound healing was scored by newly formed collagen structure, TGF β increase and epidermal and dermal regeneration, granulation tissue thickness, angiogenesis (new vessel formation). Prunus spinosa L. promotes wound healing rates with granulation tissue increase, epidermal regeneration and angiogenesis. In addition there was a significant increase in collagen and TGF [beta] ratios in immunohistochemical staining. At the end of the 4 and 10 days after treatment, a significant increase was observed in wound contraction and re-epithelialization in both Prunus spinosa and madecassol treated groups compared to the control and vehicle group. Our findings support the beneficial effects of Prunus spinosa for augmenting wound healing. The anti-inflammatory, antioxidant and antibacterial activities of Prunus spinosa increased collagen synthesis and decreased the number of inflammatory cells during wound healing and may be useful for treating skin wounds.

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