Abstract:

The aim of this study is to characterize endophytic bacteria (EB) isolated from various cultures and to identify those that have antagonistic effects against certain plant pathogens. For this purpose, 191 endophytic bacteria (EB) have been isolated from the roots, bodies, decay and leaf tissues undergoing intensive surface sterilization of the samples taken during the fide and vegetative period from some cultures widely grown in the Van Valley and around. The PGPR markers of these obtained isolates are in-vitro characteristics in terms of nitrogen fixation ability, phosphate solubility, aminosyclopropan-1-carboxylate deaminase (ACC-d) production, indol-3-acetic acid (IAA) production and siderofor production. 58.9% of the isolat is gram (-), 41% of the isolat. It is estimated that 1 is a gram (+). V33K1, V35Y1, V34G1, V17G2, V30Y3, V38K1, V17K1 and V36Y2 code EB is found to be active in all other categories except nitrogen fixation. 25 isolates in 3 different categories, 59 isolates in 2 different categories, 67 isolates show activity in only 1 category, while 21 isolates did not show activity in any category. Also, V30Y3 and V30G2 coded isolates have antagonistic effect against plant pathogens 7 different bacterial isolates. With the 16s rDNA series analysis results of the selected isolates according to the considered rating points and antagonistic effects, V40K2's Bacillus velezensis (MN186863), V30Y3's Bacillus megaterium (MN187955), V30G2's Pseudomonas caspiana (MN128080), V31Y4's Pantoea sp. (MT249279) and V33K2 Bacillus sp. It is confirmed to be MT249109.

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