Abstract:

The method of polymerase chain reaction (PCR) in real-time was used to analyze the quantitative characteristics of normal and potentially pathogenic aerobic/facultative-anaerobic and anaerobic biota in the urethra, cervical channel and vagina in healthy women aged 10–40. The biota of all the women under 40 years and some of women older than 40 was mostly represented by lactobacilli. Microbialcomposition of the biocenosis in some women older than 40 is characterized by reduction in quantity of lactobacilli and their replacement by anaerobic microorganisms, mainly, such as Atopobium vaginae (16%), Gardnerella vaginalis (12%), Megasphaera spp. (8%), Dialister spp. (8%), Eubacterium spp. (8%) and Porphyromonas spp. (4%). Rarely, a wide range of other pathogens plays its role, including inter alia: Trichomonas vaginalis (8%), Mycoplasma genitalium (4%), Neisseria gonorrhoeae (2%) andChlamydia trachomatis (1%). The most frequent are the following strain associations: A. vaginae andG. vaginalis,Eubacterium spp. andPorphyromonas spp.,U. (urealyticum + parvum) andM. genitalium,N. gonorrhoeae andCh. trachomatis,U. (urealyticum + parvum) andCandida spp. This is connected with anatomical and physiological characteristics of genitals, hormonal and immune system action. Quantitative study of the biota of urogenital tract in Dnipropetrovsk women with the use of real-time PCR is the sensitive method for diagnosing both physiological and pathological changes, and dysbiotic disorders at early stages and preventing their further development into more serious forms.

Keywords: