Abstract:

Radiation-induced liver and kidney toxicity are among the major complications of radiation applications used in cancer treatment. Radiation damage that occurs in tissues and/or organs depends on the radiation dose recei-ved. In this study, it was aimed to evaluate the possible toxic effects of ionizing radiation (IR) given in different doses on rat liver and kidney tissue. Investigation the possible effects of different doses of ionized radiation (IR) on liver and kidney tissues. In this study, 40 female Wistar albino rats were randomly divided into 4 groups. The groups were deter-mined as the control group, 1 Gy radiation group, 5 Gy radiation group and 10 Gy radiation group. Radiation applicati-ons were performed as a single dose of total body irradiation using gamma rays. Liver and kidney tissues of the groups were evaluated histopathologically through Hematoxylin-Eosin (H&E), Masson trichrome and Periodic acid Schiff (PAS) staining. Furthermore, the number of apoptotic cells was determined by caspase-3 immunostaining. When liver tissues treated with IR were examined histologically, various damaged cellwere observed in the irradiated liver tissues, especi-ally vascular and interstitial hemorrhage areas, and also the degree of damage was directly proportional with the radia-tion dose. Compared to the control group, the most prominent fibrosis was observed in the group treated with 10 Gy radiation. Furthermore, glycogen content depletion of hepatocytes was seen in 5 Gy and 10 Gy groups. The IR applied caused changes and cellular damage in the renal tubules and interstitial tissue. Especially in the 5 Gy and 10 Gy IR applied groups, the amount of collagen fibers increased and proximal tubule epithelial cells were found to have thicke-ning of the epithelial basement membranes with marked loss of microvilli. The caspase-3 + cell count showed that IR have an apoptosis-enhancing effect on both liver and kidney tissues. As a result, the study findings revealed that the tissue damage and apoptotic cell number in the liver and kidney are dependent on the dose administered in the IR.

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