Abstract:

In this paper, the effects on secondary metabolite accumulation of brassinosteroid (BR) (24-epibrassinolide (24-eBL) on immobilized cells that were obtained from Vitis vinifera cv. Cinsault was investigated. 24-eBL was treated to immobilized cells covered calcium alginate beads at concentrations of 0, 0.25, 0.50, 0.75 and 1.0 mg L-1 for one month. As a result of this study, it was found that 24-eBL applications modified secondary metabolite accumulation and had positive effects on secondary metabolite production when the suitable concentration was used. While the highest total phenolic, catechin, p-coumaric acid and chlorogenic acid contents were found in immobilized cells treated 0.75 mg L-1 24-eBL, the highest epicatechin, quercetin, trans-resveratrol contents were obtained in immobilized cells treated 0.50 mg L-1 24-eBL and the highest gallic acid content was determined in immobilized cells treated 0.25 mg L-1 24-eBL. Moreover, the highest 24-eBL concentration (1 mg L-1) decreased the content of secondary metabolite compared to the control (0 mg L-1 24-eBL) except total phenolic and catechin content. To conclude, 0.50 and 0.75 mg L-1 24-eBL concentrations were the most suitable concentrations for immobilized cell culture to provide the highest secondary metabolite accumulation.

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