Abstract:

Metastasis is among the most important factors in the decrease in breast cancer survival and treatment failure. It is of great importance to elucidate the molecular pathways of breast cancer metastasis. Recent research shows that polyphenolic compounds in plants have anti-invasive and anti-metastatic activities. Molecular mechanism of black grape extract including abundant polyphenols on breast cancer and metastasis are not yet fully known. In our study, it was aimed to determine the effect of black grape extract applied to human breast cancer cell line on cell viability and adhesion and on MMP-9 gene expression, which is thought to have a role in metastasis. In the study, cell viability by trypan blue method, cell adhesion with XTT method and MMP-9 gene expression by qRT-PCR were determined in MCF-7 breast cancer cell lines. In our study, it was found that black grape extract decreased MMP-9 gene expression, cell viability and adhesion (p> 0.05). The study results support the hypothesis that natural anti-cancer agents such as polyphenols can prevent cancer from occurring and spreading. However, further studies are needed to elucidate the molecular mechanisms of polyphenols.

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