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Çalışmanın amacı, keten tohumu katkılanmış diyet tüketiminin N-Metil N- Nitrosoure (MNU) ile oksidatif stres oluşturulmuş farelerde antioksidatif etkilerinin belirlenmesidir. Farelere tek doz (50 mg/kg) MNU intraperitonal enjeksiyon ile uygulanmıştır. Çalışmada ağırlıkları ortalama 20 (±2 gr) gram, 8 haftalık 60 adet dişi fare (Mus musculus, BALB/c) altı gruba ayrılarak çeşitli formlarda beslenmiştir. Gruplar; Kontrol (standart pellet), Grup 1 (1. hafta 50 mg/kg i.p. MNU + standart pellet /12 hafta), Grup 2 (1. hafta 50 mg/kg i.p. MNU + %10 keten tohumu içeren pellet/12 hafta), Grup 3 (İlk 6 hafta standart pellet + 6. haftada 50 mg/kg i.p. MNU + son 6 hafta %10 keten tohumu içeren pellet), Grup 4 (%10 keten tohumu içeren pellet/12 hafta), Grup 5 (%10 oranında keten bitkisi tohumu pellet/12 hafta + 6. haftada 50 mg/kg i.p MNU) olarak belirlenmiştir. Keten tohumu, standart fare diyetine %10 oranında katılarak, farelere oral yolla (ad libitum) verilmiştir. On iki haftalık uygulama sonunda servikal dislokasyon uygulanmış ve karaciğer dokuları seçilmiş biyokimyasal belirteçleri (AST, ALT, LDH, GST, GR, GPX, CAT, CaE ve EROD) değerlendirmek üzere alınmıştır. Tüm gruplarda LDH aktivitesinde kontrole göre istatistiksel olarak anlamlı bir azalma belirlenmiştir (p<0.05). CaE aktivitesi 1., 2. ve 4. gruplarda önemli düzeyde artış göstermiştir. Ayrıca, 3., 4. ve 5. grupların ALT aktivitesindeki değişimlerin kontrol grubuna göre istatistiksel olarak anlamlı olduğu belirlenmiştir (p<0.05). Sonuç olarak tek başına verilen keten tohumunun farelerde olumsuz bir etkiye sebep olmadığı, biyobelirteçlerden elde edilen sonuçların MNU etkisini yansıttığı ve keten tohumunun söz konusu etkiyi azaltıcı etkisinin olmadığı düşünülmektedir.
The aim of the study is to identify the antioxidant effects of dietary consumption of keton seed contributed to N-Methyl N-Nitrosoure (MNU) and oxidative stress created in mice. A single dose (50 mg/kg) is administered by MNU intraperitonal injection. In the study, the weights were divided in an average of 20 (±2 grams) grams, 60 female mice (Mus musculus, BALB/c) in six groups for 8 weeks, in various forms. Groups; Control (standard pellet), Group 1 (1 week 50 mg/kg i.p. MNU + standard pellet /12 weeks), Group 2 (1 week 50 mg/kg i.p. MNU + 10 % keten seed-containing pellet/12 weeks), Group 3 (the first 6 weeks standard pellet + 6 weeks 50 mg/kg i.p. MNU + last 6 weeks has been defined as pellet that contains 10% cotton seed), Group 4 (pellet that contains 10% cotton seed/12 weeks), Group 5 (pellet that contains 10% cotton plant seed/12 weeks + 6 weeks 50 mg/kg i.p MNU). The keten seed is given to the mice by oral (ad libitum) with a 10% participation in the standard mouse diet. At the end of the twelve-week application, cervical dislocation was applied and the liver tissues were taken to evaluate selected biochemical indicators (AST, ALT, LDH, GST, GR, GPX, CAT, CaE and EROD). A statistically significant reduction in LDH activity in all groups has been observed according to control (p<0.05). CaE activity increased significantly in groups 1, 2 and 4. Also, 3, 4 and 5. Changes in the ALT activity of the groups have been determined to be statistically meaningful according to the control group (p<0.05). As a result, it is believed that the seed of keten given alone does not cause a negative effect on mice, the results obtained from biobelirtecs reflect the MNU effect and the seed of keten does not have a reducing effect.
The aim of the study was to determine the antioxidant effects of the consumption of flaxseed as a dietary supplement in mice exposed to N-methyl N-nitrosourea (MNU) as an oxidative stress agent. For this aim, 60 female mice (Mus musculus, BALB/c), 8 weeks old, weighing an average of 20 (±2 g) grams, were divided into six experimental groups and fed in various forms. A single dose (50 mg/kg) of MNU was administered by intraperitoneal injection. Groups were determined as the control group (standard pellet), Group 1 (1st week 50 mg/kg ip MNU + standard pellet/12 weeks), Group 2 (1st week 50 mg/kg ip MNU + 10% flaxseed pellet/12 weeks), Group 3 (first 6-week standard pellet + 6 weeks 50 mg/kg ip MNU + Last 6 weeks 10% flaxseed pellet), Group 4 (10% flaxseed pellet/12 weeks), and Group 5 (10% flaxseed pellet/12 week + 50 mg/kg ip MNU at 6th weeks). Flaxseed was added to the standard mice diet at a rate of 10% and administered orally (ad libitum). At the end of the 12th week, cervical dislocation was applied to the mice and their liver tissues were taken to evaluate the selected biochemical markers (AST, ALT, LDH, GST, GR, GPX, CAT, CaE, and EROD). There was a statistically significant decrease in LDH activity in all groups compared to the control (p<0.05). The increase in CaE activity in groups 1, 2, and 4 was found statistically significant (p<0.05). Also, the alterations in ALT activity in groups 3, 4, and 5 were found statistically significant (p<0.05). In conclusion, the results from the biomarkers suggest that the giving of flaxseed alone did not cause a negative effect in mice, while MNU was toxic and flaxseed did not reduce the MNU effect.
Alan : Fen Bilimleri ve Matematik
Dergi Türü : Uluslararası
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