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Pectinase Production with Waste Materials by Local Water Isolate of Aspergillus fumigatus Strain 2101 and Purification and Characterization of the Enzyme ​
2016
Journal:  
Hacettepe Journal of Biology and Chemistry
Author:  
Abstract:

B u çalışmada, pektin liyaz PL yerel su kaynaklarından izole edilen Aspergillus fumigatus 2101 suşundan elde edildi. PL, amonyum sülfat çöktürmesi, jel filtrasyon ve iyon değişim kromatografisi kullanılarak saflaştırıldı. Bu saflaştırma basamaklarından sonra, 12.4 saflaştırma katsayısı elde edildi. PL’nin optimum pH ve sıcaklığı sırasıyla 8.0 ve 40˚C’de 70 dakikadır. PL’nin molekül ağırlığı 11.5 kDa’dur. Sıvı besi ortamında maksimum PL aktivitesi meyve pulpları ile elde edilmiştir. Stabilite deneyleri, PL’nin 4˚C’de 20 aya kadar kararlı olduğunu göstermiştir. En yüksek PL aktivitesi karbon kaynağı olarak sitrus pectin kullanıldığında elde edilmiştir. PL, FeSO, FeCl ve askorbik asit tarafından güçlü bir şekilde aktive edilmiştir

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Hacettepe Journal of Biology and Chemistry

Field :   Fen Bilimleri ve Matematik

Journal Type :   Uluslararası

Metrics
Article : 779
Cite : 657
2023 Impact : 0.025
Details
Hacettepe Journal of Biology and Chemistry