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Carotenoids take special attention due to their strong antioxidant properties. In the present study, the antioxidant and antiproliferative effects of carotenoids extracted from Rhodobacter sphaeroides O.U. 001 were investigated. For this, R. sphaeroides O.U. 001 was first cultivated in a 5 L bioreactor. After that, the carotenoids were extracted from R. sphaeroides O.U. 001 using acetone as solvent. The effects of carotenoid extract on proliferation of vincristine and paclitaxel resistant cells (MCF-7/Vinc, MCF-7/Pac) were investigated by MTT cytotoxicity assay. The checkerboard plate technique was performed to evaluate the interactions between carotenoid extract and cancer drugs on the cells. The antioxidant activity of the carotenoid extract was assessed by 2, 2 - diphenyl, 1- picryl hydrazyl (DPPH) radical scavenging assay. As a result of extraction, considerable amount of carotenoid yield was obtained (80.69 mg carotenoid/g dry cell weight). It was found that carotenoid extract had no inhibitory effects on cell proliferation and interaction of carotenoids with anticancer drugs exhibited antagonistic effects. In addition to these, the DPPH antioxidant activity (IC50 value) of the carotenoid extract was calculated as 25 µg/mL. In conclusion, biotechnologically produced bacterial carotenoids were shown to have considerable antioxidant activity without any cytotoxicity on cancer cells.
Carotenoids draw attention due to their strong antioxidant properties. In this study, Rhodobacter sphaeroides O.U. The antioxidant and antiproliferative effects of carotenoids extracted from 001 have been studied. For this, R. Sphaeroides O.U. 001 was multiplied in a 5L bioreactor. Later, carotenoids were extracted from R. sphaeroides O.U.001 using acetone as a solvent. The effects of carotenoid extract on the proliferation of vinkristin and paclitaxel-resistant cells (MCF-7/Vinc, MCF-7/Pac) were studied by the MTT cytotoxicity test. The effect of the interaction between carotenoid extract and cancer medications on cells was assessed by the Dama Table Plate technique. The antioxidant activity of the carotenoid extract was determined by the method of determining the radical damping activity of 2, 2 - difenil, 1-picril hydrazil (DPPH). As a result of the extraction, a significant amount of carotenoid yield was achieved (80.69 mg carotenoid/g dry cell weight). Carotenoid extract has not been found to have an inhibitor effect on cell proliferation and its interaction with anti-cancer drugs is antagonistic. In addition, the resulting carotenoid extract’s DPPH antioxidant effect (IC50 value) was calculated at 25 μg/mL. As a result, biotechnologically produced bacterial carotenoids have been shown to have significant antioxidant activity in cancer cells without having a cytotoxic effect.
Karotenoidler güçlü antioksidan özelliklerinden dolayı dikkat çekmektedirler. Bu çalışmada, Rhodobacter sphaeroides O.U. 001'den ekstrakte edilen karotenoidlerin antioksidan ve antiproliferatif etkileri araştırıldı. Bunun için, R. sphaeroides O.U. 001 önce 5 L'lik bir biyoreaktörde çoğaltıldı. Daha sonra, çözücü olarak aseton kullanılarak R. sphaeroides O.U.001'den karotenoidler ekstrakte edildi. Karotenoid ekstraktının, vinkristin ve paklitaksele dirençli hücrelerin (MCF-7/Vinc, MCF-7/Pac) proliferasyonuna etkileri MTT sitotoksisite testi ile araştırıldı. Karotenoid ekstraktı ve kanser ilaçları arasındaki etkileşimin hücreler üzerindeki etkisi dama tahtası plaka tekniği ile değerlendirildi. Karotenoid ekstraktın antioksidan aktivitesi ise 2, 2 - difenil, 1-pikril hidrazil (DPPH) radikal süpürücü aktivite tayin yöntemi ile belirlendi. Ekstraksiyon sonucunda, önemli miktarda karotenoid verimi elde edildi (80.69 mg karotenoid/g kuru hücre ağırlığı). Karotenoid ekstraktının hücre proliferasyonu üzerinde inhibitör etkisinin olmadığı ve antikanser ilaçlarla etkileşiminin antagonistik olduğu bulundu. Bunlara ek olarak, elde edilen karotenoid ekstraktının DPPH antioksidan etkisi, (IC50 değeri) 25 µg/mL olarak hesaplandı. Sonuç olarak, biyoteknolojik yolla üretilen bakteriyel karotenoidlerin, kanser hücrelerinde sitotoksik etki göstermeden önemli antioksidan aktiviteye sahip oldukları gösterilmiştir.
Alan : Fen Bilimleri ve Matematik; Mimarlık, Planlama ve Tasarım; Mühendislik; Ziraat, Orman ve Su Ürünleri
Dergi Türü : Uluslararası
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