Özet:

Today, increasing obesity is one of the preventable health problems. In obese individuals, the risk of kidney disease increases approximately 1.5 times compared to non-obese individuals. Leptin is an adipokind, which is dissolved from the fat tissue cells in proportion to the amount of fat tissue and is known to lead to an increase in free oxygen radicals in the tissue. In this study, giving leptin to rats was aimed at investigating the effect of hyperleptinemine on kidney histomorphology. Tools and Methods: 16 Wistar albino rat weighs between 250 gr and 350 gr. The rats were divided into two groups, each of which had eight animals. Group 1 – Control (intraperitoneal = i.p. Serum Physiological = SF) 7 days, 2. The Group of Leptin (i.p. 0.1 mg/kg leptin) for 7 days, planned to be. I.P for 7 days. The animals given leptin and the animals in the control group given SF for the purpose of creating the same stress were sacrificed under anesthesia at the end of the experiment and both the kidneys of all rats were removed to be examined. On the kidneys fixed in the 10% neutral form, a routine histological procedure was applied. Hematoxylene-Eosin = H-E painted cuts were evaluated under a light microscope. Results: In the control group cuts, the kidney tissues were observed in normal histological structure. Compared to the control group, in the kidney tissue samples of the leptin group, mononucleary cell infiltration in the cortex, tubular dilatation in the medula, degeneration and hemorrhagic areas, vacuular degeneration in the glomerules, proximal and distal tubular dilatation were followed. Discussions: Showing the relationship between leptin and kidney damage is important to protect against the harmful effects of obesity with hormonal and behavioral arrangements. As a result of this research, we can say that leptin affects the kidney tissue negatively and causes damage to the kidney tissue.In our opinion, the leptin factor should be taken into account when the etiology of kidney damage observed in obese individuals is explained. Keywords: Leptin, kidney, obesity, kidney damage An investigation on the effect of leptin on histomorphology of rat kidney Paradise Ak 1, Wish Bayram 2, Ilkay Armagan 2, A. Cihangir Uguz 3 1 Department of Physiology, School of Medicine, Suleyman Demirel University, Isparta, Turkey 2 Department of Histology and Embryology, School of Medicine, Suleyman Demirel University, Isparta, Turkey 3 Department of Biophysics, School of Medicine, Suleyman Demirel University, Isparta, Turkey. Abstract Aim: increasing day by day obesity, one of the preventable health problems. Obese individual increases the risk of renal disease one and a half times more compared to the non-obese individuals. Leptin is a type of adipokin hormone which is released from fat tissue cells in proportion to the amount of fat tissue. It is known that leptin leads to a rise in reactive oxygen radicals in the tissue. This study aimed at investigating if leptin affects renal tissue by giving leptin to the rats. Material - Method: The total of 16 Wistar albino rats (weights ranged from 250g-300g) in the study were randomly divided into groups of eight in two different cages. The first group was the Control group (intraperitoneally = i.p. 0.9% normal saline) and the second group was the leptin group (and 0.1 mg/kg leptin). The subjects, which were given leptin i.p. for 7 days, and those in the control group, which were given 0.9% normal saline to trigger the same stress, were sacrificed and their kidneys were taken out for examination. The kidneys were fixed with 10% neutral form was formaldehyde to routine histological procedure. The sections stained with hematoxylin-eosin = H-E were analyzed under light microscopy. Results: The sections in the control group were found to be normal histologically. Compared to these, the renal tissue samples in the leptin group were determined to have mononuclear cell infiltration in the cortex, tubular dilatation in the medulla, degeneration and hemorrhagic areas, vacuolar degeneration in glomeruli and dilation in proximal and distal tubular. Discussions: Revealing the effects of leptin on renal damage is important for avoiding harmful effects of obesity through hormonal and behavioral arrangements. This study suggested that leptin could cause renal pathologies by damaging renal tissue. We are of the opinion that being able to explain renal damage etiology exactly will be useful to differential diagnosis and treatments. Keywords: leptin, kidney, obesity, renal damage

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