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Bu çalışmada, Afyonkarahisar İli'nde satışa sunulan markasız, paketsiz kefir örneklerindeki laktik asit bakterilerinin izolasyonu, tanımlanması ve patojen test mikroorganizmaları üzerindeki antimikrobiyel etkinliği değerlendirilmiştir. 5 farklı kefir örneğinden 6 adet laktik asit bakterisi izole edilmiş ve izolatların moleküler tanımlanması için 16S rDNA PZR analizi yapılmıştır. İzolatların 16S rDNA sekans analiz sonuçlarına göre, izole edilen bakteriler; Lactobacillus fermentum, Enterococcus faecium veEnterococcus durans türleri olarak tanımlanmıştır. Laktik asit bakteri izolatlarının patojen test mikroorganizmaları üzerindeki antimikrobiyel aktivitesini belirlemek için agar spot ve agar kuyu difüzyon testi kullanılmıştır. Tüm izolatlar, test edilen patojen mikroorganizmalar üzerinde farklı seviyelerde antimikrobiyel etki göstermiştir. Laktik asit bakteri izolatları, Klebsiella pneumoniea NRRLB 4420, Pseudomonas aeuroginosa ATCC 11778, Listeria monocytogenes ATCC 1911 üzerinde diğer test mikroorganizmalarına (Staphylococcus aureus ATCC 25923, Streptococcus feacalis, Escherichia coli ATCC 35218, Bacillus subtilis NRS-744) göre daha yüksek antimikrobiyel etki göstermiştir..
In this study, the anti-microbial effectiveness of the isolation, identification and anti-microbial effectiveness of lactic acid bacteria in unbranded, unpacked kefir samples presented for sale in the Afyonkarahisar Province was evaluated. From 5 different kefir samples, 6 lactic acid bacteria were isolated and 16S rDNA PZR analysis was performed for molecular identification of insulates. According to the results of the 16S rDNA sequence analysis of isolates, the isolated bacteria are defined as Lactobacillus fermentum, Enterococcus faecium and Enterococcus durans. Lactic acid bacterial insulates have been used to determine the antimicrobial activity on the pathogenic test microorganisms and the agar spot and agar tree diffusion test. All insulates have shown antimicrobial effect at different levels on the patogenic microorganisms tested. Laktic acid bacterial isolates, Klebsiella pneumoniea NRRLB 4420, Pseudomonas aeuroginosa ATCC 11778, Listeria monocytogenes ATCC 1911 had higher antimicrobial effect than other test microorganisms (Staphylococcus aureus ATCC 25923, Streptococcus feacalis, Escherichia coli ATCC 35218, Bacillus subtilis NRS-744) .
In this study, lactic acid bacteria were isolated from unmarked, unpacked kefir samples that sold in Afyonkarahisar province, and their antimicrobial activity on pathogen test microorganisms were evaluated. 6 lactic acid bacteria were isolated from 5 different kefir samples and 16S rDNA PCR analysis was performed for molecular identifications of these isolates. According to results of 16S rDNA PCR analysis; isolated bacteria from kefir samples were identifed as Lactobacillus fermentum, Enterococcus faecium and Enterococcus durans. Agar spot and agar-well diffusion assay methods were used to determine the antimicrobial activity of the lactic acid bacteria isolates on pathogen test microorganisms. All of the isolates showed antimicrobial activity at different levels on the tested pathogenic microorganisms. Lactic acid bacteria isolates showed higher antimicrobial activity on Klebsiella pneumoniea NRRLB 4420, Pseudomonas aeuroginosa ATCC 11778, Listeria monocytogenes ATCC 1911 than other test microorganisms (Staphylococcus aureus ATCC 25923, Streptococcus feacalis, Escherichia coli ATCC 35218, Bacillus subtilis NRS-744).
Field : Fen Bilimleri ve Matematik; Ziraat, Orman ve Su Ürünleri
Journal Type : Ulusal
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