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Road transport is one of the main pollutants of atmospheric air and soil in modern cities, therefore, an important issue is the comprehensive diagnosis of the state of the environment, which can be carried out by phytoindication methods, and which make it possible to comprehensively assess the environmental risks of the roadside environment for living organisms. The article analyzes the morphological and physiological changes of Taraxacum officinale Web. that occurred under the influence of vehicle emissions. For research, we chose a territory with significant aerotechnogenic loads due to both stationary sources of pollution and traffic flows –the southwestern district of the city for the analysis of plants, the following indicators were studied: mass of plants; leaf length;proportion of altered forms; ascorbic acid content; chlorophyll concentration. In the study, biometric, spectrophotometric and statistical methods were used. The results of the studies showed that in all plants inhibition of parameters was observed in comparison with the control samples. An analysis of the tightness of the relationship between the morphophysiological parameters of the plant, the traffic intensity and the distance from the road were influenced by the average mass of plants (r=0.97) in the area with heavy traffic, the content of chlorophyll (r=0.92) in the same area, the proportion of altered forms (r=-0.94). The correlation coefficient of the proportion of altered forms is negative, since the number of altered forms decreases with increasing distance from the road. It was found, that content of chlorophyll depends on traffic intensity and the distance from the road (the correlation coefficient ranged from 0.88–0.97). Indicators of leaf length and vitamin C content turned out to be less informative, since there is no clear relationship between these indicators and distance from the road (r=0.54), although with an increase in the distance from the highway, there is a slight increase in leaf length, but the content of vitamin C did not show depending on the distance to the road. The further developments lies in the refinement of the information content of such an indicator as the content of ascorbic acid in order to use it to indicate invisible changes in the vegetation cover. Key words: Motor transport; Plant mass; Leaf length; Modified forms; Ascorbic acid; Chlorophyll References Aksu, A. (2015). Sources of metal pollution in the urban atmosphere (A case study: Tuzla, Istabul). Journal of Environmental Health Science and Engineering, 13 (1), 79. DOI: 10.1186/s40201-015-0224-9. Ashraf, M., Harris, P.J.C. (2013). Photosynthesis under stressful environments: An overview. Photosynthetica, 51(2), 163–190. DOI: 10.1007/s11099-013-0021-6. Assadi, A., Pirbalouti, A., Malekpoor, F., Teimori, N., Assadi, L. (2011). Impact of air pollution on physiological and morphological characteristics of Eucalyptus camaldulensis Den. Journal of Food, Agriculture and Environment, 9, 676–679. DOI:10.1234/4.2011.2188. Akram, N.A., Shafiq, F., M.Ashraf. (2017). Ascorbic Acid-A Potential Oxidant Scavenger and Its Role in Plant Development and Abiotic Stress Tolerance. Frontiers in Plant Science, 8, 613. DOI:10.3389/fpls.2017.00613. Alamri, S.A., Siddiqui, M.H., Al-Khaishany, M.Y., Khan, M.N., Ali, H.M., Alaraidh, I.A., Alsahli, A.A., Al-Rabiah H., Mateen M. (2017). Ascorbic acid improves the tolerance of wheat plants to lead toxicity. Journal of Plant Interactions, 13 (1). Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5405147/. DOI:10.1080/17429145.2018.1491067. Aznagulova, A.V., Kurkin, V. A., Ryzhov, V. M., Tarasenko, L.V. (2014). Anatomo-morfologicheskoe issledovanie nadzemnoj chasti oduvanchika lekarstvennogo (Taraxacum Officinale Wigg.) [Anatomical and morphological study of the aerial part of dandelion (Taraxacum Officinale Wigg.)]. Medicinskij almanah, 3 (33), 173–179 (In Russian). Balasooriya, B., Samson, R., Mbikwa, F., Vitharana, U., Boeckx, P., Meirvenne, M. (2009). Biomonitoring of urban habitat by anatomical and chemical leaf characteristics. Environmental and Experimental Botany, 65(2–3), 386–394. DOI:/10.1016/j. Bell, J.N., Honour, S.L., Power, S.A.(2011). Effects of vehicle exhaust emissions on urban wild plant species. Environmental Pollution, 159(8–9),1984-90. DOI: 10.1016/j.envpol.2011.03.006. Bessonova, V. P., Ponomaryova, O. A. (2017). Morphometric characteristics and the content of plastid pigments of the needles of Picea pungens depending on the distance from the highways. Biosystems Diversity, 25(2), 96–101. DOI:10.15421/011714. Bini C., Wahsha M., Fontana S., Maleci L. (2012). Effects of heavy metals on morphological characteristics of Taraxacum officinale Web growing on mine soils in NE Italy. Journal of geochemical exploration, 123, 101–108. DOI: 10.1016/j.gexplo.2012.07.009. Chemerys, I., Hryb, Y. (2005). Ekolohichna otsinka stanu navkolyshn'oho seredovyshcha metodamy fitoindykatsiyi [Environmental assessment of the state of the environment by phytoindication methods]. Visnyk Natsional'noho universytetu vodnoho hospodarstva ta pryrodokorystuvannya, 1 (29), 3–11(in Ukrainian). Chemerys, I., Zrazhevs'kyy, S. (2009). Vplyv vidprats'ovanykh haziv avtotransportu na fotosyntetychnu funktsiyu roslyn [Effect of vehicle exhaust on the photosynthetic function of plants]. Pytannya bioindykatsiyi ta ekolohiyi, 14 (2), 86–100 (in Ukrainian). Chemerys, I., Zahoruyko, N., Konyakin S.M. (2013). Fitomonitorynh vykydiv avtotransportu v umovakh mis'koho seredovyshcha [Phytomonitoring of vehicle emissions in urban environment]. Lyudyna i dovkillya: problemy neoekolohiyi, 3–4, 141–146 (in Ukrainian). Dániel, P., Kovács, B., Prokisch, J., Gyõri, Z. (1997). Heavy metal dispersion detected in soils and plants alongside roads in Hungary. Chemical Speciation & Bioavailability, 9(3), 83–93. DOI: 10.1080/09542299.1997.11083292. Dolatabadian, A., Jouneghani, R.S. (2009). Impact of Exogenous Ascorbic Acid on Antioxidant Activity and Some Physiological Traits of Common Bean Subjected to Salinity Stress. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 37 (2), 165–172. DOI:10.15835/nbha3723406. El-Khatib, A.A., El-Shanawany, A.A., El-Amery, E.M. (2016). Urban tree leaf as bio-indicator for air pollution around superphosphate fertilizers plant, Upper Egypt. Journal of Ecology of Health & Environment An International Journal, 4 (2), 95–101. DOI:10.18576/jehe/040207. Erofeeva, E.A. (2014). Dependence of dandelion (Taraxacum officinale Wigg.) seed reproduction indices on intensity of motor traffic pollution. Dose Response, 12(4), 540–550. DOI: 10.2203/dose-response.14-009. Fröhlichová, A., Száková, J., Najmanová, J., & Tlustoš, P. (2018). An assessment of the risk of element contamination of urban and industrial areas using Taraxacum sect. Ruderalia as a bioindicator. Environmental Monitoring and Assessment, 190(3), 150. DOI: 10.1007/s10661-018-6547-0. Ga?rca-Gil, J.C., Plaza C., Soler-Rovira P., & Polo A. (2000). Long-term effects of municipal solid waste compost application on soil enzyme activities and microbial biomass. Soil Biology & Biochemistry, 32, 1907–1913. DOI:10.1016/S0038-0717(00)00165-6. Giacomino, A., Malandrino, M., Colombo, M.L., Miaglia, S., Maimone, P., Blancato, S., Conca, E., Abollino O. (2016). Metal content in dandelion (Taraxacum officinale) leaves: influence of vehicular traffic and safety upon consumption as food. Journal of Chemistry, 2016. Retrieved from https://www.hindawi.com/journals/jchem/2016/9842987/ DOI:10.1155/2016/9842987. Gupta, B., Huang, B. (2014). Mechanism of Salinity Tolerance in Plants: Physiological, Biochemical, and Molecular Characterization. Internftional Journal of Genomics,1, 1-18. DOI: 10.1155/2014/701596. Hossain, M.A., Munné-Bosch, S., Burritt, D.J., Diaz-Vivancos, P., Fujita, M., Lorence A. (2017). Ascorbic Acid in Plant Growth, Development and Stress Tolerance. DOI:10.1007/978-3-319-74057-7. Ianovici, N. (2016). Taraxacum officinale (Asteraceae) in the urban environment: seasonal fluctuations of plant traits and their relationship with meteorological factors. Acta Agrobotanica, 69 (3). Retrieved from https://pbsociety.org.pl/journals/index.php/aa/issue/view/482/showToc. DOI:10.5586/aa.1677. Iqbal, M.Z., Shafig, M., Zaidi, S.Q., Athar M. (2015). Effect of automobile pollution on chlorophyll content of roadside urban trees. Global journal of environmental science and management publication, 1(4), 283–296. DOI: 10.7508/gjesm.2015.04.003. Kabata-Pendias, A. (2011). Trace Elements in Soil and Plants. CRC Press, Boca Raton, FL. Kabata-Pendias, A., Dudka, S. (1991).Trace metal contents of Taraxacum officinale (dandelion) as a convenient environmental indicator. Environmental geochemistry and health, 13 (2), 108–130. DOI: 10.1007/BF01734301. Keane, B., Collier, M.H., Rogstad, S.H. (2005) Pollution and genetic structure of North American populations of the common dandelion (Taraxacum officinale). Environmental Monitoring and Assessment, 105(1–3), 341–357. DOI:10.1007/s10661-005-4333-2. Khan, T.A., Mazid, M., Mohammad F. (2011). A review of ascorbic acid potentialities against oxidative stress induced in plants, Journal of agrobiology, 28(2), 97–111. DOI: 10.2478/v10146-011-0011-x. Ková?ik, J., Dudáš, M., Hedbavny, J., Mártonfi, P. (2016). Dandelion Taraxacum linearisquameum does not reflect soil metal content in urban localities. Environmental pollution, 218, 160–167. DOI: 10.1016/j.envpol.2016.08.030. Kudryavs`ka, T.B., Dychko, A.O. (2014). Metod ocenki i prognozirovaniya vliyaniya tehnogennogo zagryazneniya na vozduh urboe`kosistemy [Method for assessing and predicting the impact of industrial pollution on the air of urban ecosystems]. Vostochno-evropeyskiy zhurnal peredovyh tehnologiy. T.1, 10 (67 ), 4–7. DOI: 10.15587/1729-4061.2014.19882 (In Russian). Kolesnikov, S., Kazeev, K., Val'kov, V. (2001). Biojekologicheskie principy monitoringa i normirovanija zagrjaznenija pochv [Bioecological principles of monitoring and regulation of soil pollution]. CVVR, Rostov-n/D. (In Russian). Labuzova O.M., Noskova T.V., Lysenko M.S., Ilina E.G. (2016). Ekoanaliticheskij kontrol i bioindikaciya sostoyaniya gorodskoj territorii [Ecological control and bioindication of urban area]. Acta Biologica Sibirica, 2(3), 21–24. DOI: 10.14258/abs.v2i3.1451 (In Russian). Ligocki, M., Tarasewicz, Z., Zygmunt, A., Ani??ko, M. (2011). The common dandelion (Taraxacum officinale) as an indicator of anthropogenic toxic metal pollution of environment. Acta Scientiarum Polonorum. Zootechnica, 10 (4), 73–82. Lugovskaya, A.Yu., Khramova, E.P., Trubina, L.K. (2014). Effect of transport and industrial pollution on morphological and biochemical parameters of Potentilla fruticosa (Rosaceae). Rastitel`nyy mir aziatskoy Rossii, 1, 71–76. Maslennikov, P.V., Chupakhina, G.N., Skrypnik, L.N., Feduraev, P.V., Melnik, A.S. (2018). Assessment of the Antioxidant Potential of Plants in Urban Ecosystems under Conditions of Anthropogenic Pollution of Soils. Russian Journal of Ecology. Vo. 49, 5, 384–394. DOI: 10.1134/S1067413618050065. Maslennikov, P.V., Chupakhina, G.N., Skrypnik, L.N., Feduraev P.V., Melnik, A.S. (2018). The contribution of polyphenols to plantresistance to Pb soil pollution. International Journal of Environmental Studies, 75 (5), 719–731. DOI: 10.1080/00207233.2018.1440816. Markert, B., Wünschmann, S., Baltr??nait?? E. (2012). Innovative observation of the environment. Bioindicators and biomonitors: definitions, strategies and applications. Journal of Environmental Engineering and Landscape Management, 20 (3), 221–239. DOI: 10.3846/16486897.2011.633338 Mikalaj?n??, A., Jaku?ionyt??, L. (2011). Investigation into heavy metal concentration by the gravel roadsides. Journal of Environmental Engineering and Landscape Management, 19 (1), 89–100. DOI: 10.3846/16486897.2011.557474 Musiyenko, M.M., Parshikova, T.V., & Slavnij, P.S. (2001). Spektrofotometrichni metodi v praktici fiziologiyi, biohimii ta ekologiyi roslin [Spectrophotometric methods in the practice of physiology, biochemistry and plant ecology]. Phytosociocenter, Kyev (in Ukrainian). Myslyuk, O., Myslyuk, Ye., Solomka, L. (2010). Otsinka vplyvu vykydiv Cherkas'koyi TETs na stan urbolandshaftiv [Assessment of the impact of Cherkasy HEP emissions on the state of urban landscaping]. Visnyk ONU. Khimiya. Vo.15 (12–13), 47–53 (in Ukrainian). Myslyuk, ??., Khomenko, ??., Yehorova, ??. (2019). Ekolog??chna oc??nka kislotno-osnovnih vlastivostej urbozem??v m. Cherkasi [Ecological as sessment for the acid-base properties of urban soils in Cherkasy city]. Transactions of Kremenchuk Mychailo Ostrohradskyi National University, 4, 117. DOI: 10.30929/1995-0519.2019.4.53-59/ (in Ukrainian). Myslyuk ??., Khomenko ??., Yehorova ??., Pydorenko V. (2019). Ekolog??chn?? aspekti stanu urbozem??v [Environmental aspects of urban soils]. Visnyk ?herkaskogo derzhavnogo technolohichnogo universytetu. Seria: Tehnichni nauky, 2, 123–133. DOI: 10.24025/2306-4412.2.2019.172233) (in Ukrainian). Nagajyoti, P.C., Lee, K. D., Sreekanth, T.V.M. (2010). Heavy metals, occurrence and toxicity for plants: a review. Environmental Chemistry Letters, 8, 199–216. DOI:10.1007/s10311-010-0297-8. Nersisyan, G. S., Hovhannisyan H. A. (2010). Application of Phytoindication Method for Controlling Air Pollution in Yerevan. Armenia. Journal of Life Sciences, Vo. 4, 4 (29), 52–57. DOI:10.17265/1934-7391/2010.06.010. Normandin, L., Kennedy, G., Zayed, J. (1999). Potential of dandelion (Taraxacum officinale) as a bioindicator of manganese arising from the use of methylcyclopentadienyl manganese tricarbonyl in unleaded gasoline. Science of the total environment, 239(1–3), 165–71. DOI:10.1016/s0048-9697(99)00292-2. Pehlivan, F.E. (2017). Vitamin C: An Antioxidant Agent. Retrieved from https://www.intechopen.com/books/vitamin-c/vitamin-c-an-antioxidant-agent. DOI: 10.5772/intechopen.69660. Pozolotina, V.N. (2003). Otdalennye posledstviya dejstviya radiacii na rasteniya [Longterm effects of radiation on plants]. Akademkniga, Ekaterinburg (In Russian). Raina, A.K., Chand Bala (2011). Effect of vehicular pollution on Duranta repens L. in Jammu City. Journal of Applied and Natural Science 3 (2), 211–218. DOI:10.31018/jans.v3i2.181. Regional'na dopovid' pro stan navkolyshn'ogo pryrodnogo seredovyshha v Cherkas'kij oblasti u 2018 roci [Regional report on the state of the environment in Cherkasy region in 2018] (2019). Cherkassy (in Ukrainian). Samancioglu, A., Sat, I.G., Yildirim, E. (2016). Total phenolic and vitamin C content and antiradical activity evaluation of traditionally consumed wild edible vegetables from Turkey. Indian Journal of Traditional Knowledge, 15 (2), 208–213. DOI:10.1080/01448765.2013.827992. Sanchez, R.A. (1967). Some Observations about the Effect of Light on the Leaf Shape in Taraxacum Officinale L. Retrieved from http://edepot.wur.nl/299514. Simon, L., Martin, H.W., Adriano D.C. (1996). Chicory (Cichorium intybus L.) and dandelion (Taraxacum officinale Web.) as phytoindicators of cadmium contamination. Water, Air, and Soil Pollution, 91, 351–362. DOI:10.1007/bf00666269. Smirnoff, N. (2018). Ascorbic acid metabolism and functions: A comparison of plants and mammals. Free Radical Biology & Medicine, 122, 116–129. DOI: 10.1016/j.freeradbiomed.2018.03.033. Smirnoff, N., Wheeler, G.L. (2000). Ascorbic acid in plants: biosynthesis and function. Critical Reviews in Biochemistry and Molecular Biology, 35(4), 291–314. DOI: 10.1080/10409230008984166. Spencer, H.J., Scott, N.E., Port G.R., Davison A.W. (1988). Effects of roadside conditions on plants and insects. I. Atmospheric conditions. Journal of applied ecology, 25 (2), 699–707. DOI: 10.2307/2403855. Tesliyk, A. ?. (2017). Environmental Assessment of the State of Atmospheric Air of Commercial Holdings by Bioindicating Methods. Scientific Bulletin of UNFU, 27(6), 85–88. DOI: 10.15421/40270617. Tsandekova, O.L., Neverova, O.A., Kolmogorova, E.Yu. (2013) The role of antioxidant system in the stability of pine stands on a coal spoil bank. Izvestiya Samarskogo nauchnogo centra Rossiyskoy akademii nauk, 15 (3), 559–562. Tyulkova, ??.G. (2015) Morphological parameters leaves woody plants in an urban environment (on Gomel example). Ecology and noospherology. Vo. 26, 3–4, 62–71. DOI: 10.15421/031520. Wen-yi, Qu, Chun-kui, Hu (2012). Xibei nonglin keji daxue xuebao. Ziran kexue ban [Contact colors of the leaves of plants and ecological parameters of the environment, specifies a method for wireless communications]. Jornal Northwest A&F University Natural Science, 7, 221–228 (in Chinese). Wilson, M.A., Burt, R., Indorante, S.J., Jenkins, A.B., Chiaretti, J.V., Ulmer, M.G., Scheyer J.M. (2008). Geochemistry in the modern soil survey program. Environment Monitoring Assessment, 139, 151–171. DOI:10.1007/s10661-007-9822-z. Youssef, N., Markert B., Gurbanov, E., Sevnic, H., Wünschmann, S. (2014). Bioindication of trace metal pollution in the atmosphere of Baku city using ligustrum japonicum, olea europea, and pyracantha coccinea leaves. Journal of Environmental Engineering and Landscape Management, 22 (1), 14–20. DOI:10.3846/16486897.2013.804828. Zhang, F., Yan, X., Zeng, C., Zhang, M., Shrestha, S., Devkota, L.P., Yao, T. (2012). Influence of Traffic Activity on Heavy Metal Concentrations of Roadside Farmland Soil in Mountainous Areas. International journal of environmental research and public health, 9, 1715–1731. DOI:10.3390/ijerph9051715. Zhou X., Sun, C., Zhu, P., F, Liu. (2018). Effects of Antimony Stress on Photosynthesis and Growth of Acorus calamus. Frontiers in Plant Science, 9, 579. DOI: 10.3389/fpls.2018.00579.

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