Özet:

Grapevine leafroll disease (GLRD) is one of the most important viral diseases of grapevine worldwide. The aim of this study was to investigate the prevelance of Grapevine leafroll associated virus-1 (GLRaV-1), Grapevine leafroll-associated virus-2 (GLRaV-2), Grapevine leafroll-associated virus-3 (GLRaV-3), Grapevine leafroll-associated virus 4 (GLRaV-4), and Grapevine leafroll-associated virus-9 (GLRaV-9) in Tokat province of Turkey. In 2018, leaf samples and one-year-old shoots of plants, showing virus-like symptoms, were collected from different vineyards in Central, Erbaa, Niksar, and Pazar districts of Tokat province. Totally 197 samples were obtained and tested molecularly using two-step polymerase chain reaction (PCR) by using virus-specific primers for GLRaV-1, GLRaV-2, GLRaV-3, GLRaV-4) and GLRaV-9. At least 106 (53.80%) samples were found to be infected showed single or multiple infections. The 103 (52.28%) samples resulted in positive with GLRaV-1 while the infection rates were %5.18 for GLRaV-3 with 10 samples, %2.53 for GLRaV-4 with 5 samples, %1.03 for GLRaV-2 and GLRaV-9 with 2 samples, respectively. The amplified PCR products of GLRaV-1, GLRaV-2 and GLRaV-3 were sequenced, aligned with MEGAX, and analyzed in BLAST for multiple comparisons of other isolates in the GenBank. Sequence analysis of viruses showed 94-96 % , 96-99 % and, 99-100 % nucleotide identity with references isolates in the GenBank for GLRaV-1, GLRaV-2, and UO3-3 isolate of GLRaV-3 respectively.