Özet:

Background: In this study, we aimed to evaluate the presence of oxidative stress in chronic kidney disease (CKD) by measuring dynamic thiol/disulphide homeostasis. Material and Method: A hundred fifty patients (57 men, 93 women) followed with a diagnosis of CKD (stages 1-5, not on dialysis) and 76 healthy, disease-free individuals (30 men, 46 women) were included in the study. Blood thiol/disulphide homeostasis was measured by newly developed automatic and colorimetric method by Erel and Neselioglu. Results: The average serum native thiol (p = 0.001), total thiol (p = 0.001) and disulphide (p = 0.041) levels were lower in CKD than in the control group. No significant difference was found between the CKD and control groups in terms of disulphide/thiol, disulphide/total thiol and thiol/total thiol ratio (p > 0.05). Serum native thiol and total thiol levels were correlated negatively with both BMI and serum phosphorous level while both the parameters were correlated positively with glomerular filtration rate, total protein level and albumin level. A negative correlation was found between total thiol and age. Disulphide levels were correlated positively with albumin and total protein and negatively with uric acid levels. Conclusion: Our study shows that low serum thiol levels in CKD is due to a decrease in total thiol reserve of the body and not due to the conversion of thiol groups into disulphides.

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