Özet:

Objective: Hyper IgE Syndrome (HIES) and Common Variably Immunodeficiency (CVID) are immuno-deficiency diseases. HIES is characterized by recurrent skin abscesses, pneumonia, mucocutaneous fungal infections, eczama, eosinophilia and high serum IgE levels. CVID is characterized by recurrent bacterial infections in airways and gastrointestinal tract. In this study, differentiation of Th17 cells were aimed to be investigated in CVID and HIES patients. Method: Two groups of patients diagnosed either with HIES and CVID and one group including four healty individuals were enrolled into the study. In each group, peripheral blood mononuclear cells (PBMC) and CD45RA+ naive T cells were isolated from venous blood. Isolated cells were cultured in Th17 differentiating conditions and IL-17 levels of culture supernatants were measured by ELISA method. Results: When naive T cells obtained from HIES patients were cultured under Th17 differentiating conditions, culture supernatant IL-17 cytokine level did not show any significant increase compared to unstimulated group. When PBMCs isolated from the same patients were cultured under differentiating conditions, IL-17 cytokine level had been measured nearly statistical significant compared to healthy control group. On the other hand, when naive T cells isolated from CVID and healthy control groups were cultured under Th17 differentiating conditions, a significant increase had been observed in IL-17 levels of both groups compared to the unstimulated group. Results of differentiated cultures of isolated PBMC and naive T cells showed that there is no significant difference in the IL-17 cytokine levels between the healthy group and CVID. Conclusion: These results show that there may be a defect in IL-17 secreting T cells in HIES group, but there is no defect in IL-17 secreting T cells in CVID patients.

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