Abstract:

Purpose: Our country has a rich flora and many medicinal and aromatic plants within it. Today, phytotherapy research has become popular. The aim of this study is to evaluate the antioxidant and antimicrobial capacities of methanolic extrain derived from the plant Dactylorhiza osmanica, the effect of thyrozinase inhibitors. The antioxidant properties of the extrain were studied using in vitro antioxidant determination methods such as total phenolic content (TPC), iron-reducing antioxidant power (FRAP) and the 1,1-difenyl-2-pikrilhydrazil (DPPH) radical spray activity test. The phenolic composition of the extract was studied with high-performance fluid chromatography (RP-HPLC) in the reverse stage. Antimicrobial activity was tested using the disk diffusion method. The activity of the thyrozinase inhibitors was colorimetrically measured. Results: Extrain’s TPC, FRAP, and DPPH activities were found at 20.6 ± 0.3785 mg gallic acid equivalent/gram sample, 804 ± 8.6217 μM Trolox/g sample and 0.1838 ± 0.0015 mg/mL. Gallic acid, protokatekuic aldehide, protokatekuic acid, p-hydroxy benzoic acid, chlorogenic acid, vanilic acid, caffeic acid, vaniline, shiringaldehyde, p-cumaric acid, ferolic acid, sinapic acid, benzoic acid are phenolic compounds found in the extract. In the determination of the activity of the thyrozinase inhibitors, the extrenin IC50 value was not found meaningful. Extreme, acid-fast bacteria (M. smegmatis), some gram-positive (S. aureus and B. cereus) and some gram-negative (Y. pseudotuberculosis) bacteria showed medium antibacterial activity. Result: According to the results of this study, D. osmanica can be considered as a potential source in the development of new pharmaceuticals.

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