Özet:

Alternative in vitro nanotechnological methods have been developed to conventional methods in the treatment strategies of breast cancer. Silver nanoparticles (AgNPs) from metallic nanoparticles, especially those amplified using the green synthesis method, show promise as a suitable anticancer candidate in the field of nanomedicine. The purposed of the investigation was to green synthesize and characterize of silver nanoparticles (So-AgNPs) by Salvia officinalis aqueous extract and evaluate their anticancer effect on metastatic breast cancer cell line MCF-7. The formation of So-AgNPs were characterized by scanning electron microscopy (SEM) with Energy dispersive X-ray (EDX) and UV-visible spectroscopy. The surface absorption peak of So-AgNPs was observed at 417 nm by the UV-Vis analysis. MCF-7 and CRL-4010 cells were treated with different concentrations (0-0.05 µg/mL) of AgNPs for 24 h. The cytotoxic effect of green synthesized AgNPs against MCF-7 and CRL-4010 cell lines was approved by MTT [3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyl tetrazolium bromide] assay. MTT results showed that So-AgNPs have higher cytotoxic activity in MCF-7 breast cancer cell lines than in CRL-4010 human breast epithelial cells (IC50 values 8.49 and 9.69 µg/mL, respectively). AgNPs synthesized via Salvia officinalis extract exhibited a significant cytotoxic effect on MCF-7 cell lines, demonstrating that they could be a potential antitumor agent in the treatment of metastatic breast cancer. However, further research is required to elucidate the mechanisms of action.

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