Abstract: The volatile oil composition of Scolymus hispanicus L. was investigated. Essential oil of aerial parts were obtained through hydro-distillation and determined with GC-MS analyses. Fifteen compounds were determined in the oil (99.3%) of aerial part. The main compounds were heneicosane (19.4 %), hexahydrofarnesly acetone (17.0%) and phytol (17.0%). Saturated n-alkane derivatives (35.2%), oxygenated sesquiterpenes (25.6%) and diterpene (17.0%) were dominated in the oil. Also, the antibacterial activity of volatile oil was studied against Escherichia coli and Staphylococcus aureus bacteria. But the oil did not show activity against the tested microorganisms at 80-10 mg/ mL concentrations. Here, it is reported for the first time on the volatile oil composition of S. hispanicus.
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