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Amaç: Paraoksonaz-1 (PON1) HDL yapısında bulunan, HDL ve LDL’yi oksidasyondan koruyarak aterosklerotik lezyonlardaki oksidatif stresi azaltan antioksidan bir enzimdir. Bu çalışmanın amacı, sağlıklı kişilerin oral trigliserid tolerans testine (OTTT) verdikleri cevaba göre PON1 enziminin paraoksonaz, arilesteraz ve laktonaz aktivitelerini değerlendirmektir. Gereç ve Yöntem: Gönüllüler, yaşları 18-55 arasında değişen 45 kadın ve 51 erkek olmak üzere toplam 96 sağlıklı bireyden oluşmaktadır. Gönüllüler, açlık ve OTTT sonrası 2, 4 ve 6’ncı saatlerdeki TG seviyeleri kullanılarak hesaplanan eğri altındaki alan (AUC) değerlerine göre üç farklı gruba ayrılmıştır. PON1 enzim aktiviteleri ve diğer parametreler OTTT cevabı düşük olan grup ile yüksek olan grup arasında karşılaştırıldı. PON1 enzim aktiviteleri spektrofotometrik metodlarla belirlendi. Bulgular: Erkeklerde üst grup ile alt grup karşılaştırıldığında aterojenik lipid profili, artmış total kolesterol ve LDL-K ile azalmış HDL-K düzeyleri gözlemlendi. PON1 laktonaz aktivitesi erkeklerde kadınlara göre anlamlı düşük bulundu (P<0.05). PON1 laktonaz aktivitesi her iki cinste de OTTT süresince zamana bağlı olarak artış gösterdi. Kadınlarda, üst grupta PON1 arilesteraz aktivitesi alt gruba göre anlamlı yüksek bulundu (P<0.022). Sonuç: OTTT cevabı yüksek olan üst grupların aterojenik lipit profiline sahip oldukları gözlemlenmiştir. PON1 enzim aktivitelerinin oksidatif strese bir cevap olarak postprandial dönemde genellikle artış eğiliminde olduğu, ayrıntılı çalışmalara ihtiyaç duyulduğu düşünülmüştür.
Purpose: Paraxonase-1 (PON1) is an antioxidant enzyme found in the HDL structure, which reduces oxidative stress in atherosclerotic lesions by protecting HDL and LDL from oxidation. The aim of this study is to evaluate the activity of the enzyme PON1 paraoxonase, arylesterase and lactonase according to the response given by healthy people to the oral triglyceride tolerance test (OTTT). Volunteers are composed of a total of 96 healthy individuals, including 45 women and 51 men aged between 18 and 55. Volunteers are divided into three different groups according to curved area (AUC) values calculated using TG levels in the 2, 4 and 6 hours after hunger and OTTT. PON1 enzyme activities and other parameters were compared between the group with low OTTT response and high group. PON1 enzyme activity was determined by spectrophotometric methods. Results: At men compared to the upper group to the lower group, atherogenic lipid profile, increased total cholesterol and LDL-K levels were observed with HDL-K levels decreased. The activity of PON1 lactonase was significantly lower in men compared to women (P<0.05). PON1 lactonase activity in both sexes increased depending on time during the OTTT period. In women, the activity of PON1 arylesterase in the upper group was significantly high compared to the subgroup (P<0.022). Result: Top groups with high OTTT response have been observed to have an atherogenic lipite profile. The activity of the PON1 enzyme is considered to tend to increase in the postprandial period as a response to oxidative stress, requiring detailed studies.
Aim: Paraoxonase-1 (PON1) whic is present in HDL structure, is an enzyme that decreases the oxidative stress in atherosclerotic lesions by preventing the HDL and LDL against to ox-idation. Thu study aims to determine the paraoxonase, arylesterase and lactonease activities of PON1 enzyme, taking into account the response of healthy subjects to the oral triglyceride tolerance test (OTTT). Material and Method: Study group included 96 healthy subjects (45 female and 51 male with age range of 18-55 years). Study group was divided into three groups according to the area under curve (AUC) values calculated by using triglyceride levels at the fasting state and at 2nd, 4th and 6th hours after the high fat diet (OTTT). PON1 enzyme activity levels were determined by spectrofotometric methods. PON1 enzyme activities and other parameters in lower OTTT response group were compared to that of the upper group. Results: Atherogenic lipid profile, increased total cholesterol and LDL-C and decreased HDL-C levels, was observed in subjects with the upper group men when compared to the lower group. PON1 lactonase activity was significantly lower in men than that of women (P<0.05). On the other hand, PON1 lactonase activity showed time-depended increase during OTTT in both sex. PON1 arylesterase activity in subjects with the upper group was significantly higher than that of the lower groups (P<0.022) in women. Conclusion: It was observed that the upper groups with high OTTT response had an atherogenic lipid profile. It has been thought that PON1 enzyme activities tend to increase in the Postprandial period as a response to oxidative stress and detailed studies are needed.
Alan : Sağlık Bilimleri
Dergi Türü : Uluslararası
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