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Çalışmamızda Eylül -Aralık 2020 tarihlerinde 20 çiğ süt, 20 peynir, 20 çiğ tavuk eti, ve 20 çiğ et olmak üzere 80 adet gıda ürününden izole edilen Enterococcus izolatlarının tanımlanması için temel biyokimyasal testler ve identifikasyon işlemi MALDI-TOF Kütle Spektrofotometresi ile yapılarak 60 izolattan 42 (%70) Enterococcus faecalis ve 18 (%30) Enterococcus faecium türü izole edilmiştir. E.faecalis ve E.faecium türlerinin Kirby-Bauer disk difüzyon metoduyla antibiyotik direnç profilleri, lipaz, jelatinaz, DNaz aktiviteleri, hemolitik aktivitesi, S tabakası varlığı ve biyofilm aktiviteleri incelenmiştir. E.faecalis türlerinin en fazla duyarlı olduğu antibiyotikler %100 kloramfenikol, streptomisin, vankomisin ve teikoplanin, %97,6 gentamisin ve norfloksasin, %95,2 ampisilin, %92,8 siprofloksasin olarak saptanmıştır. En fazla dirençlilik gösterdiği antibiyotik %90,4 tetrasiklindir. E.faecium türlerinin en fazla duyarlı olduğu antibiyotikler %100 ampisilin, kloramfenikol, streptomisin, gentamisin, vankomisin ve teikoplanin, % 77,7 siprofloksasin, %72,2 norfloksasindir. En fazla dirençlilik gösterdiği antibiyotik %61,1 tetrasiklindir. Lipaz ve jelatinaz aktiviteleri tümünde negatif, DNaz aktiviteleri ise tümünde pozitif sonuç vermiştir. S tabakası varlığı E.faecalis’te %83,3 ve E.faecium’da %16,6 bulunmuştur. Biyofilm aktiviteleri Kongo Red Agar’da 60 adet Enterococcus spp. türü bakterinin 2‘si (%3.3) kuvvetli, 22‘si (%36.6) orta kuvvetli, 36’sı (%60) zayıf biyofilm pozitif üretmiştir. Mikroplak yönteminde E.faecalis’te 32’si (%53,3) zayıf, 8’i (%13,3) orta kuvvetli, 2’si (%3,3) kuvvetli biyofilm üretmiştir. E.faecium’da 17’si (%28,3) zayıf, 1’i (%1,6) orta kuvvetli biyofilm üretmiştir. Kuvvetli biyofilm üreten Enterococcus türleri saptanmamıştır.
In our study in September-December 2020, the basic biochemical tests and identification process for the identification of Enterococcus isolates, which were isolated from 20 raw milk, 20 cheese, 20 raw chicken meat, and 20 raw meat, from 80 food products, made by the MALDI-TOF Mass Spectrophotometry, 42 (70%) and 18 (30%) types of Enterococcus faecium were isolated from 60 isolates. With the Kirby-Bauer disc diffusion method of E.faecalis and E.faecium species, antibiotic resistance profiles, lipase, jelatinase, DNaz activities, hemolithic activity, S layer presence and biophilm activities have been studied. and E. The most sensitive antibiotics of the faecalis species were determined as 100% chloramfenicol, streptomycin, vancomycin and thyroplanin, 97.6% gentamisin and norfloxacin, 95.2% ampisilin and 92.8% siprofloksacin. The antibiotic with the highest resistance is 90.4% tetracycline. The most sensitive antibiotics of E.faecium are 100% ampisillin, chloramfenicol, streptomycin, gentamisin, vancomycin and thyroplanin, 77.7% siprofloksacin, 72.2% norfloksacin. The antibiotic with the highest resistance is 61.1 % tetrasicillin. Lipaz and jelatinaz activities have been negative in all, while DNaz activities have been positive in all. The S layer presence was 83.3% in E.faecalis and 16.6% in E.faecium. Biophilic activities in the Congo Red Agar 60 pieces of Enterococcus spp. 2 types of bacteria (3.3%) are strong, 22 (36.6%) are medium-strong, 36 (60%) are weak and produced biophilic positive. In the microplate method, E.faecalis produced 32 (53.3%) weak, 8 (13.3%) medium-strong, 2 (3.3%) strong biophilms. In E.faecium, 17 (28.3%) produced weak, 1 (1.6%) produced medium-powered biofilms. There are no kinds of enterococcus that produce strong biophiles.
In our study, basic biochemical tests and identification process for the identification of Enterococcus isolates isolated from 80 food products, including 20 raw milk, 20 cheese, 20 raw chicken meat, and 20 raw meat, between September and December 2020, were performed with MALDI-TOF Mass Spectrophotometer and 42 of 60 isolates were identified. (70%) Enterococcus faecalis and 18 (30%) Enterococcus faecium species were isolated. Antibiotic resistance profiles, lipase, gelatinase, DNase activities, hemolytic activity, presence of S layer and biofilm activities of E.faecalis and E.faecium species were investigated by Kirby-Bauer disc diffusion method. The antibiotics to which E.faecalis species were most susceptible were found to be 100% chloramphenicol, streptomycin, vancomycin and teicoplanin, 97.6% gentamicin and norfloxacin, 95.2% ampicillin, 92.8% ciprofloxacin. The antibiotic with the highest resistance is 90.4% tetracycline. The antibiotics to which E.faecium species are most susceptible are 100% ampicillin, chloramphenicol, streptomycin, gentamicin, vancomycin and teicoplanin, 77.7% ciprofloxacin, 72.2% norfloxacin. The antibiotic with the highest resistance is 61.1% tetracycline. Lipase and gelatinase activities were negative in all, and DNase activities were positive in all. Presence of S layer was found 83.3% in E.faecalis and 16.6% in E.faecium. Biofilm activities of 60 Enterococcus spp. on Congo Red Agar. Of the bacteria, 2 (3.3%) were strong, 22 (36.6%) were medium strong, and 36 (60%) were weak biofilm positive. In the microplate method, 32 (53.3%) weak, 8 (13.3%) medium-strong, 2 (3.3%) strong biofilms were produced in E.faecalis. In E.faecium, 17 (28.3%) produced weak biofilms and 1 (1.6%) produced medium-strength biofilm. Strong biofilm-producing Enterococcus species were not detected.
Alan : Fen Bilimleri ve Matematik; Mühendislik
Dergi Türü : Uluslararası
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