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  Citation Number 5
 Views 20
 Downloands 4
Bir Alabalık Çiftliğinde Doğal Enfekte Gökkuşağı Alabalıklarından (Oncorhynchus mykiss Walbaum, 1792) Lactococcus garvieae’nin Kültür ve PCR ile Saptanması ve Etkenin Antibiyotik Duyarlılık Profillerinin Belirlenmesi
2015
Journal:  
Veterinary Sciences and Practices
Author:  
Abstract:

Bu olgu sunumunda, Karadeniz Bölgesi’nde enfeksiyon çıkan bir ticari gökkuşağı alabalığı çiftliğinden toplanan 8 adet balık örneği incelendi. Hastalık etkeni saptandı ve etkenin antibiyotik duyarlılık profili araştırıldı. Salgın 21 oC su sıcaklığında ve 2014 yılı Ağustos ayı içerisinde görüldü. Enfeksiyon bir aylık süre içerisinde % 70-75 oranında mortaliteye neden oldu. Bakteriyolojik incelemeler sonucunda 4 balığın karaciğer, dalak ve böbreklerinden Tryptic Soy Agar, McConkey Agar ve Shotts-Waltman Agar’da Lactococcus garvieae izole edildi. Suşların identifikasyonu kültür tekniği ile konfirmasyonu ise moleküler yöntemlerle yapıldı. Çalışmada elde edilen izolatların fenotipik özelliklerinin birbirleriyle uyumlu olduğu saptandı. Suşların tamamı florfenikol, eritromisin, oksitetrasiklin, amoksisilin ve ampisiline duyarlı, trimetoprim, sulfametoksazol/trimetoprim ve okzolinik asite dirençli bulundu. Enrofloksasin, sefoperazon, kanamisin, neomisin, ve gentamisine karşı değişen oranlarda duyarlılık belirlenirken suşların tamamında çoklu antibiyotik direnci tespit edildi.

Keywords:

From the natural infected cranberry cranberry cranberry (Oncorhynchus mykiss Walbaum, 1792) Lactococcus garvieae with culture and PCR and the determination of the effect’s antibiotic sensitivity profiles
2015
Author:  
Abstract:

In the presentation of this fact, eight samples of fish collected from a commercial cloudy alabali farm that was infected in the Black Sea Region were studied. The disease factor was identified and the antibiotic sensitivity profile of the factor was studied. It was observed at 21 °C water temperature and in August 2014. The infection caused a 70-75% mortality within a month period. The bacteriological trials resulted in the insulation of the liver, waves and kidneys of 4 fish in Tryptic Soy Agar, McConkey Agar and Shotts-Waltman Agar. The identification of the shells and the confirmation of the culture by means of molecular methods. The study found that the phenotypical properties of the insulates were compatible with each other. All of the suges were found resistant to florphenol, erythromycin, oxythrasiclin, amoxicillin and ampicillin, trimetoprim, sulfametoxazole/trimetoprim and oxolic acid. Sensitivity to enrofloxacin, sephoperazone, kanamisine, neomycin, and gentamisine was determined in variable rates, while multiple antibiotic resistance was detected in all the acids.

Keywords:

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Veterinary Sciences and Practices

Journal Type :   Ulusal

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Veterinary Sciences and Practices