Abstract:

Cancer constitutes one of the greatest threats to human health. Considering that drug resistance is one of the main problems in cancer treatment, studies on the effects of natural compounds on cancer cells are increasing rapidly. Edible mushrooms are considered as a functional and healthy food source because they are rich in nutritional values. Studies have shown that they have anticancer, anti-inflammatory, antioxidant, and antimicrobial effects. Tricholoma fracticum (Britzelm.) Kreisel, which grows naturally in many regions of our country and in the province of Elazığ, is an edible mushroom consumed as food. In this study, it was aimed to determine the cytotoxic effect of methanol extract of T. fracticum at different concentrations (1000µg/mL, 500 µg/mL, 250 µg/mL, 125 µg/mL and 62.5 µg/mL) against Hep3B cells at 24 and 48 hours. 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method was used to determine the cytotoxic effect. In the results obtained, the methanol extract of T. fracticum showed a significant cytotoxic effect at a concentration of 1000 µg/mL at the 24th (1.97±0.65%) and 48th hour (0.61±0.27%). The IC50 value of the methanol extract of the fungus against the Hep3B cell line was calculated as 166.76±7.65 µg/mL at 24 hours and 134.38±5.45 µg/mL at 48 hours. We think that the cytotoxic results of T. fracticum extract against Hep3B cell line may be important and this species, which is less studied among Tricholoma species, should be studied more and brought to the literatüre.