Abstract:

Purpose: Dietylnitrozamine (DEN) and phenobarbital (PB) are produced by reactive oxygen types (ROS) during metabolism. Ellagic acid (EA) is a biologically active, natural polyphenol with antioxidant activity. This study was done to evaluate the preventive and protective effects of EA on the liver damage caused by DEN-PB in mice. The tool and method: 60 adult Sprague-Dawley rats were divided into 6 groups: Negative control, DEN, EA, DEN-PB (positive control), DEN-PB-EA (treatment) and EA-DENPB (pre-treatment). DEN, positive control, treatment and pre-treatment groups were given 150 mg/kg DEN. Two weeks later, 500 ppm PB was applied to positive control and treatment groups. EA and the pre-treatment groups were given 50 mg/kg/day EA orally for eight weeks and the treatment group for four weeks. Eight weeks later, the rats were decaptivated, the liver and blood were subjected to biochemical assessment. The findings: it was found that DEN alone or with PB significantly increased the levels of MDA in the liver, but it did not change the plasma levels of MDA (p<0,001). EA pre-treatment has reduced the levels of MDA in the liver. CAT activity has not changed in the liver and erythrosites of the positive control group. The hepatic CAT activity was significantly increased in pre-treatment and significantly increased in therapy (p<0,05). SOD activity, DEN and positive control groups did not show any variation in the liver and erythrosites. EA significantly increased the activity of the liver SOD (p<0,001). While the application of DEN alone or with PB significantly decreases both the liver and blood GSH levels (p<0,05), the application of EA increased the liver GSH levels in the treatment group (p<0,05). The blood GSH levels in the therapeutic group were significantly reduced (p<0,001). Recommendation: EA protective therapy is believed to be more effective than EA therapy in DEN and PB-based liver oxidative stress in mice.

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